Fig. 24.1 Schematic representation of the way how to perform IVCT. Liquid isopentane -propane cryogen (−193 °C) was directly poured over the kidneys of anesthetized rats, which were then removed…
Fig. 14.1 A flow chart of different experimental procedures, including perfusion -fixation (a), immersion-fixation (b), quick-freezing (c), and in vivo cryotechnique (d) for living mouse heart s Fig. 14.2 A…
Fig. 50.1 Immunolocalization of serum protein s in the tumor tissues formed by the Lu65 cells and prepared with cryobiopsy (Cb). Their serial sections were stained with hematoxylin-eosin (HE, a)…
Fig. 52.1 (a–l) Expression of mitochondria-targeted Dendra2 (mitoDen2, green) and mCherry (red) in cultured B16-BL6 cells shows that the fluorescence signals are significantly reduced with immersion fixation with ethanol (EtOH,…
Fig. 31.1 (a) A flow chart of the three different preparation procedures, namely, in vivo cryotechnique with freeze-substitution (i), quick-freezing of fresh resected tissues followed by freeze-substitution (ii), and conventional…
Fig. 51.1 Glycogen distribution of cryobiopsied liver tissue specimens stained with PAS . (a) Fully PAS-stained hepatocytes are clearly observed in the specimen without α-amylase treatment. (b) Hepatocytes around the…
Fig. 49.1 (a)–(c) Light micrographs of a xenograft tumor of Lu99 cells prepared with cryobiopsy show that there is little formation of ice crystal s (b, solid arrowheads) and flowing…
Fig. 45.1 Mouse lung tissues prepared by IVCT at 5 s (a), 1 min (b), and 5 min (c–f) after the melanoma cell injection. HE-stained morphological features (HE; a–d) and…
Fig. 46.1 Light micrographs of serial paraffin sections prepared by IVCT and stained with H&E (HE, a–c) or immunostained for albumin (d–f), immunoglobulin G 1 (IgG1 , g–i), or immunoglobulin…
Fig. 26.1 Scanning electron micrographs of freeze-fractured renal corpuscles of living mice under normal blood flow condition, as prepared by IVCT. (a) Interdigitating foot process es are covering the outer…
