Avian Circadian Organization
Fig. 5.1 Actogram of locomotor activity from a single zebra finch, Taeniopygia guttata. The top bars indicate the times during which lights are off (black) vs. on (white). These are…
GENERAL
Control of Rest–Activity Behavior by the Central Clock in Drosophila
Fig. 3.1 The molecular clock in Drosophila: The schematic depicts the daily cycle that drives cyclic expression of per and tim mRNAs and PER and TIM proteins. Protein expression is…
Immunohistochemical Analyses of Serum Proteins in Puromycin Aminonucleoside Nephropathy of Living Rat Kidneys
Fig. 24.1 Schematic representation of the way how to perform IVCT. Liquid isopentane -propane cryogen (−193 °C) was directly poured over the kidneys of anesthetized rats, which were then removed…
Dynamic Bioimaging of Serum Proteins in Beating Mouse Hearts
Fig. 14.1 A flow chart of different experimental procedures, including perfusion -fixation (a), immersion-fixation (b), quick-freezing (c), and in vivo cryotechnique (d) for living mouse heart s Fig. 14.2 A…
Immunolocalization of Serum Proteins in Xenografted Mouse Model of Human Tumor Cells
Fig. 50.1 Immunolocalization of serum protein s in the tumor tissues formed by the Lu65 cells and prepared with cryobiopsy (Cb). Their serial sections were stained with hematoxylin-eosin (HE, a)…
Bioimaging of Fluorescence-Labeled Mitochondria in Subcutaneously Grafted Murine Melanoma Cells by the “In Vivo Cryotechnique”
Fig. 52.1 (a–l) Expression of mitochondria-targeted Dendra2 (mitoDen2, green) and mCherry (red) in cultured B16-BL6 cells shows that the fluorescence signals are significantly reduced with immersion fixation with ethanol (EtOH,…
Immunohistochemical Detection of Angiotensin II Receptors in Mouse Cerebellum
Fig. 31.1 (a) A flow chart of the three different preparation procedures, namely, in vivo cryotechnique with freeze-substitution (i), quick-freezing of fresh resected tissues followed by freeze-substitution (ii), and conventional…
Histochemical Approach of Cryobiopsy for Glycogen Distribution in Living Mouse Livers Under Fasting and Local Circulation Loss Conditions
Fig. 51.1 Glycogen distribution of cryobiopsied liver tissue specimens stained with PAS . (a) Fully PAS-stained hepatocytes are clearly observed in the specimen without α-amylase treatment. (b) Hepatocytes around the…
Significance of Cryobiopsy for Morphological and Immunohistochemical Analyses of Xenografted Human Lung Cancer Tissues and Functional Blood Vessels
Fig. 49.1 (a)–(c) Light micrographs of a xenograft tumor of Lu99 cells prepared with cryobiopsy show that there is little formation of ice crystal s (b, solid arrowheads) and flowing…
Imaging of Thrombosis and Microcirculation in Mouse Lungs of Initial Melanoma Metastasis
Fig. 45.1 Mouse lung tissues prepared by IVCT at 5 s (a), 1 min (b), and 5 min (c–f) after the melanoma cell injection. HE-stained morphological features (HE; a–d) and…
