Fig. 52.1 (a–l) Expression of mitochondria-targeted Dendra2 (mitoDen2, green) and mCherry (red) in cultured B16-BL6 cells shows that the fluorescence signals are significantly reduced with immersion fixation with ethanol (EtOH,…
Fig. 50.1 Immunolocalization of serum protein s in the tumor tissues formed by the Lu65 cells and prepared with cryobiopsy (Cb). Their serial sections were stained with hematoxylin-eosin (HE, a)…
Fig. 31.1 (a) A flow chart of the three different preparation procedures, namely, in vivo cryotechnique with freeze-substitution (i), quick-freezing of fresh resected tissues followed by freeze-substitution (ii), and conventional…
Fig. 51.1 Glycogen distribution of cryobiopsied liver tissue specimens stained with PAS . (a) Fully PAS-stained hepatocytes are clearly observed in the specimen without α-amylase treatment. (b) Hepatocytes around the…
Fig. 49.1 (a)–(c) Light micrographs of a xenograft tumor of Lu99 cells prepared with cryobiopsy show that there is little formation of ice crystal s (b, solid arrowheads) and flowing…
Fig. 45.1 Mouse lung tissues prepared by IVCT at 5 s (a), 1 min (b), and 5 min (c–f) after the melanoma cell injection. HE-stained morphological features (HE; a–d) and…
Fig. 46.1 Light micrographs of serial paraffin sections prepared by IVCT and stained with H&E (HE, a–c) or immunostained for albumin (d–f), immunoglobulin G 1 (IgG1 , g–i), or immunoglobulin…
Fig. 26.1 Scanning electron micrographs of freeze-fractured renal corpuscles of living mice under normal blood flow condition, as prepared by IVCT. (a) Interdigitating foot process es are covering the outer…
Fig. 47.1 Light micrographs of HE- and PAS -stained liver tissues by various preparation methods. With HE-stained CB liver tissues at surface area, congested erythrocytes are observed (a: small arrows,…
Fig. 21.1 Confocal laser scanning micrographs showing the distribution of endogenous albumin (red color) and IgG (green color) in living mouse glomeruli under four different fixation methods. (a) Both albumin…
