Fig. 1 Flow graph of the described metagenomic-based approach to detect potential pathogens 3.1 Sample Preparation 1. For each hive, collect approximately 50 adult worker bees in total, from both…
Genotype Known hosts Subtypes Type strain (representative strain of subtype) GenBank acc. no. A Psittacine, pigeon, canary, turkey A-VS1 VS1, MN Zhang AF269281 pheasant, chicken, duck, cattle A-6BC 6BC X56980 …
Fig. 1 Phylogeny of the major groups (in bold) and sublineages (marked by stars) of B. anthracis (modified from [8] and [9]) Cost is an important issue for all diagnostic…
Fig. 1 MOL-PCR general scheme 2 Materials 2.1 DNA Preparation and Biosafety Procedures 1. Bacillus anthracis is a class 3 pathogen and should be manipulated in a BSL3 laboratory. Viability…
Fig. 1 Illustration of the real-time monitoring and alarm system of the IZSLER Veterinary Biobank facility 3 Sample Types and Preparation Pre-analytical conditions are a key factor in maintaining the…
Fig. 1 General workflow and timeline of the Mycoplasma and Chlamydia assays Validated protocols for differentiation among 37 Mollicutes species [10] and nine Chlamydia spp. [28] have been published by…
Fig. 1 ASFV viral DNA digested with restriction enzyme. (a) Lane 1: DNA ladder. Lane 2: two microliters (400 ng) of non-digested ASFV viral DNA. (b) Lane 1: DNA ladder….
© Springer Science+Business Media New York 2015Mónica V. Cunha and João Inácio (eds.)Veterinary Infection Biology: Molecular Diagnostics and High-Throughput StrategiesMethods in Molecular BiologyMethods and Protocols124710.1007/978-1-4939-2004-4_32 32. Functional Genomics of Tick Vectors Challenged with the Cattle…
Fig. 1 Generic location of the six segments in the target DNA used to design LAMP primers. Forward (F3) and backward (B3) outer primers and forward (FIP) and backward (BIP)…
Description 454/Roche Illumina/Solexa SOLiD Platform Genome Sequencer FLX Genome Analyzer IIx SOLiD 3 Plus System Sequencing method Emulsion PCR of bead-bound oligos Isothermal bridge amplification on flow cell Emulsion PCR…
