Proteomics Characterization of Tick-Host-Pathogen Interactions
Database search Buffer 1 Buffer 2 Buffer 3 Buffer 4 Ixodida 62.9 % 48.2 % 44.4 % 82.0 % Pecora 72.4 % 44.2 % 40.2 % 72.7 % aThe efficacy…
Fluorescence In Situ Hybridization for the Tissue Detection of Bacterial Pathogens Associated with Porcine Infections
Target species Tissue sample Systematic namea Probe sequence (5′–3′) References Actinobacillus pleuropneumoniae Lung, ear, joints, bone, brain S-S-Ap-0185-a-A-19 CCCACCCTTTAATCCATAG [11–14] Genus Brachyspira Intestine L-G-Serp-1410-a-A-19 GTCATTCCATCGAAACATA [15–17] Brachyspira hyodysenteriae Intestine L-S-S.hyo-1210-a-A-19…
Pulsed-Field Gel Electrophoresis (PFGE): Application in Population Structure Studies of Bovine Mastitis-Causing Streptococci
Fig. 1 Schematic drawing of DNA agarose discs preparation (adapted from Chung et al. [11]). Source: H. de Lencastre, ITQB. Portugal) 5. Pipette 150 μl of the OD620 = 5.0 cell…
Next-Generation Sequencing in Veterinary Medicine: How Can the Massive Amount of Information Arising from High-Throughput Technologies Improve Diagnosis, Control, and Management of Infectious Diseases?
Fig. 1 Steps in next-generation sequencing and data analysis workflows where error and bias introduction may occur Fig. 2 High-throughput technologies can be applied to numerous aspects of animal infectious…
Detection of Fish Pathogens by Loop-Mediated Isothermal Amplification (LAMP) Technique
Target virus Primer sequences (5′–3′) References VHSa F3—GGS AAG CAA GGA YCA CGA G [13] B3—CAG GTG TCC YTC TAG TGT TTC FIP—GAT CCA CCG ATA CTG TTT TTG GGG…
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs) Analysis of Members of the Mycobacterium tuberculosis Complex
Fig. 1 Schematic structure of a CRISPR/Cas region in MTBC strains and application of the CRISPR array, to the development of the spoligotyping method, using 43 selected spacer sequences. (a)…
Biological Specimen Collection and Processing for Molecular Analysis
Clinical signs Sample to submit for testing Hepatitis Liver Drop in egg production Feces/large intestines, vaginal swabs Lameness Synovial fluid, tendons, heart, liver Respiratory Tracheas, tracheal swabs, feces, cloacal swabs,…
Identification of Animal Pasteurellaceae by MALDI-TOF Mass Spectrometry
Fig. 1 MALDI-TOF spectrum of Actinobacillus pleuropneumoniae type strain S4074T The MALDI-TOF mass spectrometers designed for microbial identification purpose generally are equipped with databases for the identification of bacteria of…
Gold Nanoparticles as a Potential Tool for Diagnosis of Fish Diseases
Fig. 1 Schematic diagram of a colorimetric assay based on unmodified AuNPs for detection of unamplified nucleic acids (SVCV RNA). First, the target RNA is denatured and the complementary probe…
Validation of Molecular Diagnostic Assays and Quality Assurance and Control in the Veterinary Laboratory
Fig. 1 Assay development and validation plan In vitro diagnostics (IVDs) for human use come under statutory regulations in most jurisdictions (e.g., the US Food and Drug Administration (FDA), the…
