In Vivo 18F-FDG-PET Imaging in Mouse Atherosclerosis
Fig. 1 PET/CT scanner and different equipment needed for anesthesia, injection of CT contrast agent, and temperature control (a) CT subsystem (see Note 3 ). (b) PET subsystem (see…
SUGERY, ORTHOPEDICS & ANESTHESIA
Intravital Microscopy in the Cremaster Muscle Microcirculation for Endothelial Dysfunction Studies
Fig. 1 Mouse board for cremaster muscle intravital microscopy. The board should be maintained in a constant temperature (36.5–37.5 °C) with a heating system. There are two areas in the…
Immunostaining of Macrophages, Endothelial Cells, and Smooth Muscle Cells in the Atherosclerotic Mouse Aorta
Fig. 1 Gravity flow apparatus for vascular perfusion. Perfusant is suspended at a height of 60 cm above the mice to be perfused so as to maintain a pressure of…
Quantification of Apoptosis in Mouse Atherosclerotic Lesions
Fig. 1 Morphology of normal and apoptotic smooth muscle cell nuclei. (a) Control mouse carotid artery demonstrating normal nuclear morphology. (b) Carotid artery showing nuclear condensation (filled arrows) compared to…
Quantification of In Vitro Macrophage Cholesterol Efflux and In Vivo Macrophage-Specific Reverse Cholesterol Transport
Fig. 1 Schematic diagram of the biliary macrophage-RCT pathway. Cholesteryl esters (CE) stored in lipid droplets of macrophage foam cells are hydrolyzed by neutral cholesteryl ester hydrolases. Lipid-poor nascent preβ-migrating…
Isolation and Culture of Aortic Smooth Muscle Cells and In Vitro Calcification Assay
Fig. 1 Double-collagenase digestion procedure for VSMC isolation from aorta 3.2 Mouse Aorta Dissection 1. Select mice from which aortas will be dissected. Usually 5–10 mice are required for…
In Vitro Differentiation of Naïve CD4+ T Cells: A Tool for Understanding the Development of Atherosclerosis
Fig. 1 Immunomagnetic selection of cells. (1) The biotinylated antibodies are bound to the positive cells. (2) The streptavidin-microbeads bind to the biotinylated antibodies. (3) The magnetic columns are placed…
Magnetic Resonance Imaging of the Atherosclerotic Mouse Aorta
Fig. 1 Representative T1-weighted MR serial images showing the abdominal aorta (white arrow) above the right renal artery of an apoE-KO mouse demonstrating large atherosclerotic lesions protruding the arterial lumen….
Detection of Intraplaque Hemorrhage in Mouse Atherosclerotic Lesions
Intervention [reference] Agea (weeks) Diet Brachiocephalic artery (%) Carotid artery (%) None [3, 4] 20 WTD 8 0 Age [5] 42–50 Chow 66–75 n.d. Collar [7] 52 WTD n.a. 8…
Immunostaining of Lymphocytes in Mouse Atherosclerotic Plaque
Target antigen Host species (label) Clone Dilution Comment Mouse CD3 Hamster 500A2 1:100 Permeabilization step required Mouse CD4 Rat H129.19 1:100 Mouse CD8 Rat 53-6.7 1:100 Mouse FoxP3 Rat FJK-16s…
