Fig. 46.1 Light micrographs of serial paraffin sections prepared by IVCT and stained with H&E (HE, a–c) or immunostained for albumin (d–f), immunoglobulin G 1 (IgG1 , g–i), or immunoglobulin…

Fig. 26.1 Scanning electron micrographs of freeze-fractured renal corpuscles of living mice under normal blood flow condition, as prepared by IVCT. (a) Interdigitating foot process es are covering the outer…

Fig. 47.1 Light micrographs of HE- and PAS -stained liver tissues by various preparation methods. With HE-stained CB liver tissues at surface area, congested erythrocytes are observed (a: small arrows,…

Fig. 21.1 Confocal laser scanning micrographs showing the distribution of endogenous albumin (red color) and IgG (green color) in living mouse glomeruli under four different fixation methods. (a) Both albumin…

Fig. 23.1 Schematic drawing of experimental design and “in vivo cryotechnique ” (IVCT) used in the study [9]. Following two consecutive daily intraperitoneal injection of bovine serum albumin (BSA), kidney…

Fig. 25.1 Light micrographs of mouse kidneys prepared with the “in vivo cryotechnique ” (IVCT) or resection of fresh tissues and quick-freezing (RF-QF), followed by FS. (a, b) Serial sections…

Fig. 38.1 A flow chart showing a series of conventional preparation procedures (a), freezing (b), in vivo cryotechnique (c), and cryobiopsy (d), followed by freeze-substitution (e) and immunostaining (f) or…

Fig. 39.1 Serial sections of mouse cerebellum prepared with the conventional fixation-dehydration method and embedded in paraffin show pCREB immunoreactivity (a–d, f, g, i, j) and DAPI (e, g, h,…

Fig. 37.1 (a) A schematic drawing of “in vivo cryotechnique ” (IVCT) for living mouse sciatic nerves under various stretching conditions. A sciatic nerve of an anesthetized mouse is exposed,…

Fig. 20.1 (a) A schematic drawing of “in vivo cryotechnique ” for mouse kidneys under acute hypertensive condition . The abdominal aorta is ligated by thin thread just below both…