Fig. 41.1 (a–c) Fluorescence micrographs of quick-frozen, freeze-substituted, and freeze-dried TRITC- and FITC-conjugated goat immunoglobulins, which were mixed on glass slide from different direction (arrows in a, b). (c) is…
Fig. 22.1 Representative light micrograph (a) and confocal laser scanning micrographs (b–d) showing periodic acid-Schiff staining ( PAS ; a), its fluorescence emission ( PAS fluorescence emission; b), and immunofluorescence…
Fig. 11.1 Light micrographs of hematoxylin–eosin (HE)-stained sections of normal mouse spleen , prepared with the in vivo cryotechnique (IVCT) followed by freeze-substitution (FS). (a) Low-magnified view of the white…
Fig. 19.1 Light micrographs of mouse renal cortical tissues prepared under various hemodynamic condition s ; “in vivo cryotechnique ” under normotensive (a, normal), heart-arrest (b, heart-arrest), and acute hypertensive…
Fig. 36.1 (a–f) Differential interference contrast (DIC; a, c, e) and the glutamate immunostaining (Glu; b, d, f) images of the eyeball tissue sections with IVCT followed by FS with…
Fig. 9.1 Light microscopic images of HE staining (a, d, g, j, m, p, s, v) and immunostaining of ERK1/2 (b, e, h, k, n, q, t, w) and phosphorylated…
Fig. 35.1 (a) Flow chart of procedure with IVCT and FS for immunohistochemical detection of phosphorylated protein. Dynamically changing molecule under phosphorylation is immediately immobilized with IVCT. (b) Schematic representation…
© Springer Japan 2016Shinichi Ohno, Nobuhiko Ohno and Nobuo Terada (eds.)In Vivo Cryotechnique in Biomedical Research and Application for Bioimaging of Living Animal Organs10.1007/978-4-431-55723-4_34 34. Overview on Recent Applications of In Vivo Cryotechnique in Neurosciences Nobuhiko Ohno1 ,…
Fig. 32.1 (a) A schematic representation of “in vivo cryotechnique ” (IVCT) for the mouse cerebellum after opening the scull; cryoknife is push into the cerebellum and simultaneously isopentane –propane…
Fig. 15.1 Morphological features of mouse lungs. (a, c) Macroscopic images of normal mouse lungs under experimental expiration (Ex) and inspiration (In). (b, d) Light microscopic images with HE staining…
