© Springer Japan 2016Shinichi Ohno, Nobuhiko Ohno and Nobuo Terada (eds.)In Vivo Cryotechnique in Biomedical Research and Application for Bioimaging of Living Animal Organs10.1007/978-4-431-55723-4_54 54. Concluding Remarks Shinichi Ohno1 , Nobuhiko Ohno1 and Nobuo Terada1, 2 (1) Department of Anatomy…
Fig. 53.1 (a) Chemical structure of adenosine triphosphate (ATP) . (b, c) Schematic representation of the chemical attachment of ATP to a glass (b) or tissues (c) by the glutaraldehyde…
Fig. 29.1 Serial paraffin sections of antral follicles in the control (N; a–d, i–k, o–q, u–w) or mifepristone -induced polycystic ovary model (PCO; e–h, l−n, r–t, x–z) mouse ovaries prepared…
Advantages Limitations 1. Reduction of ischemic/anoxic artifacts by direct cryofixation 1. Preferred exposure of target organs for increasing regions without visible ice crystals 2. High time resolution by immediate freezing…
Fig. 18.1 Light micrographs of hematoxylin-eosin staining of paraffin sections of living mouse thymic tissues prepared by IVCT (a–f). (a) A low-magnification view of the thymic tissues shows widely panoramic…
Fig. 44.1 (a) Raman spectra of human whole blood at low temperature (−150 °C) under different SO2 conditions. (b) Schematic representation of Raman cryomicroscopy for living mouse intestines, prepared with…
Fig. 43.1 (a) Schematic representation of Raman microscopic analysis for the freeze-dried samples (arrow) sandwiched between glasses. (b, c) Raman mapping image (b) and morphological image with toluidine-blue staining (c)…
Fig. 42.1 (a) Photographs of different sizes of quantum dot s (QDs; 4.5, 6, and 7 nm) emitting different fluorescent colors under ultraviolet light . (b) Chemical structure of GSH-coated…
Fig. 28.1 Serial paraffin sections prepared by in vivo cryotechnique and stained with hematoxylin-eosin (HE, a) or immunostained for albumin (Alb, b), immunoglobulin G 1 (IgG1 , c), or IgM…
Fig. 40.1 Replica electron micrographs of intestinal smooth muscle cell s in living mice prepared by the IVCT followed by replica preparation method. (a) Freeze-fractured cytoplasm of three parallel-lining smooth…
