Genotype Known hosts Subtypes Type strain (representative strain of subtype) GenBank acc. no. A Psittacine, pigeon, canary, turkey A-VS1 VS1, MN Zhang AF269281 pheasant, chicken, duck, cattle A-6BC 6BC X56980 …
Fig. 1 Phylogeny of the major groups (in bold) and sublineages (marked by stars) of B. anthracis (modified from [8] and [9]) Cost is an important issue for all diagnostic…
Fig. 1 MOL-PCR general scheme 2 Materials 2.1 DNA Preparation and Biosafety Procedures 1. Bacillus anthracis is a class 3 pathogen and should be manipulated in a BSL3 laboratory. Viability…
Fig. 1 Illustration of the real-time monitoring and alarm system of the IZSLER Veterinary Biobank facility 3 Sample Types and Preparation Pre-analytical conditions are a key factor in maintaining the…
Fig. 1 ASFV viral DNA digested with restriction enzyme. (a) Lane 1: DNA ladder. Lane 2: two microliters (400 ng) of non-digested ASFV viral DNA. (b) Lane 1: DNA ladder….
Fig. 1 General workflow and timeline of the Mycoplasma and Chlamydia assays Validated protocols for differentiation among 37 Mollicutes species [10] and nine Chlamydia spp. [28] have been published by…
Fig. 1 Generic location of the six segments in the target DNA used to design LAMP primers. Forward (F3) and backward (B3) outer primers and forward (FIP) and backward (BIP)…
© Springer Science+Business Media New York 2015Mónica V. Cunha and João Inácio (eds.)Veterinary Infection Biology: Molecular Diagnostics and High-Throughput StrategiesMethods in Molecular BiologyMethods and Protocols124710.1007/978-1-4939-2004-4_32 32. Functional Genomics of Tick Vectors Challenged with the Cattle…
Description 454/Roche Illumina/Solexa SOLiD Platform Genome Sequencer FLX Genome Analyzer IIx SOLiD 3 Plus System Sequencing method Emulsion PCR of bead-bound oligos Isothermal bridge amplification on flow cell Emulsion PCR…
Primer/probe Sequence (5′–3′) Complementary target F_Actin GGC TCY ATY CTG GCC TC β–actin gene of mammalsa R_Actin GCA YTT GCG GTG SAC RAT G P_Actinb Cy5.5-TAC TCC TGC TTG CTG…
