Target species Tissue sample Systematic namea Probe sequence (5′–3′) References Actinobacillus pleuropneumoniae Lung, ear, joints, bone, brain S-S-Ap-0185-a-A-19 CCCACCCTTTAATCCATAG [11–14] Genus Brachyspira Intestine L-G-Serp-1410-a-A-19 GTCATTCCATCGAAACATA [15–17] Brachyspira hyodysenteriae Intestine L-S-S.hyo-1210-a-A-19…
Fig. 1 Schematic drawing of DNA agarose discs preparation (adapted from Chung et al. [11]). Source: H. de Lencastre, ITQB. Portugal) 5. Pipette 150 μl of the OD620 = 5.0 cell…
Fig. 1 Steps in next-generation sequencing and data analysis workflows where error and bias introduction may occur Fig. 2 High-throughput technologies can be applied to numerous aspects of animal infectious…
Target virus Primer sequences (5′–3′) References VHSa F3—GGS AAG CAA GGA YCA CGA G [13] B3—CAG GTG TCC YTC TAG TGT TTC FIP—GAT CCA CCG ATA CTG TTT TTG GGG…
Clinical signs Sample to submit for testing Hepatitis Liver Drop in egg production Feces/large intestines, vaginal swabs Lameness Synovial fluid, tendons, heart, liver Respiratory Tracheas, tracheal swabs, feces, cloacal swabs,…
Fig. 1 Schematic structure of a CRISPR/Cas region in MTBC strains and application of the CRISPR array, to the development of the spoligotyping method, using 43 selected spacer sequences. (a)…
Fig. 1 MALDI-TOF spectrum of Actinobacillus pleuropneumoniae type strain S4074T The MALDI-TOF mass spectrometers designed for microbial identification purpose generally are equipped with databases for the identification of bacteria of…
Fig. 1 Assay development and validation plan In vitro diagnostics (IVDs) for human use come under statutory regulations in most jurisdictions (e.g., the US Food and Drug Administration (FDA), the…
Fig. 1 Schematic diagram of a colorimetric assay based on unmodified AuNPs for detection of unamplified nucleic acids (SVCV RNA). First, the target RNA is denatured and the complementary probe…
Fig. 1 Flow graph of the described metagenomic-based approach to detect potential pathogens 3.1 Sample Preparation 1. For each hive, collect approximately 50 adult worker bees in total, from both…
