Fig. 25.1 Light micrographs of mouse kidneys prepared with the “in vivo cryotechnique ” (IVCT) or resection of fresh tissues and quick-freezing (RF-QF), followed by FS. (a, b) Serial sections…
Fig. 23.1 Schematic drawing of experimental design and “in vivo cryotechnique ” (IVCT) used in the study [9]. Following two consecutive daily intraperitoneal injection of bovine serum albumin (BSA), kidney…
Fig. 38.1 A flow chart showing a series of conventional preparation procedures (a), freezing (b), in vivo cryotechnique (c), and cryobiopsy (d), followed by freeze-substitution (e) and immunostaining (f) or…
Fig. 39.1 Serial sections of mouse cerebellum prepared with the conventional fixation-dehydration method and embedded in paraffin show pCREB immunoreactivity (a–d, f, g, i, j) and DAPI (e, g, h,…
Fig. 37.1 (a) A schematic drawing of “in vivo cryotechnique ” (IVCT) for living mouse sciatic nerves under various stretching conditions. A sciatic nerve of an anesthetized mouse is exposed,…
Fig. 20.1 (a) A schematic drawing of “in vivo cryotechnique ” for mouse kidneys under acute hypertensive condition . The abdominal aorta is ligated by thin thread just below both…
Fig. 17.1 Light micrographs of serial paraffin sections of living mouse thymic tissues prepared by IVCT, which are stained with H&E (a, b) or immunostained for albumin (c, d), IgG1…
Fig. 53.1 (a) Chemical structure of adenosine triphosphate (ATP) . (b, c) Schematic representation of the chemical attachment of ATP to a glass (b) or tissues (c) by the glutaraldehyde…
Fig. 33.1 Electron micrographs of molecular layers (a, b) or Purkinje cell layers (c) in mouse cerebellar cortex prepared by the “in vivo cryotechnique ” without ischemia (IVCT; a, c)…
© Springer Japan 2016Shinichi Ohno, Nobuhiko Ohno and Nobuo Terada (eds.)In Vivo Cryotechnique in Biomedical Research and Application for Bioimaging of Living Animal Organs10.1007/978-4-431-55723-4_54 54. Concluding Remarks Shinichi Ohno1 , Nobuhiko Ohno1 and Nobuo Terada1, 2 (1) Department of Anatomy…
