Target virus Primer sequences (5′–3′) References VHSa F3—GGS AAG CAA GGA YCA CGA G [13]  B3—CAG GTG TCC YTC TAG TGT TTC   FIP—GAT CCA CCG ATA CTG TTT TTG GGG…

Clinical signs Sample to submit for testing Hepatitis Liver Drop in egg production Feces/large intestines, vaginal swabs Lameness Synovial fluid, tendons, heart, liver Respiratory Tracheas, tracheal swabs, feces, cloacal swabs,…

Fig. 1 Schematic structure of a CRISPR/Cas region in MTBC strains and application of the CRISPR array, to the development of the spoligotyping method, using 43 selected spacer sequences. (a)…

Fig. 1 MALDI-TOF spectrum of Actinobacillus pleuropneumoniae type strain S4074T The MALDI-TOF mass spectrometers designed for microbial identification purpose generally are equipped with databases for the identification of bacteria of…

Fig. 1 Assay development and validation plan In vitro diagnostics (IVDs) for human use come under statutory regulations in most jurisdictions (e.g., the US Food and Drug Administration (FDA), the…

Fig. 1 Schematic diagram of a colorimetric assay based on unmodified AuNPs for detection of unamplified nucleic acids (SVCV RNA). First, the target RNA is denatured and the complementary probe…

Fig. 1 Flow graph of the described metagenomic-based approach to detect potential pathogens 3.1 Sample Preparation 1. For each hive, collect approximately 50 adult worker bees in total, from both…

Genotype Known hosts Subtypes Type strain (representative strain of subtype) GenBank acc. no. A Psittacine, pigeon, canary, turkey A-VS1 VS1, MN Zhang AF269281  pheasant, chicken, duck, cattle A-6BC 6BC X56980 …

Fig. 1 Phylogeny of the major groups (in bold) and sublineages (marked by stars) of B. anthracis (modified from [8] and [9]) Cost is an important issue for all diagnostic…

Fig. 1 MOL-PCR general scheme 2 Materials 2.1 DNA Preparation and Biosafety Procedures 1. Bacillus anthracis is a class 3 pathogen and should be manipulated in a BSL3 laboratory. Viability…