Fig. 23.1 Schematic drawing of experimental design and “in vivo cryotechnique ” (IVCT) used in the study [9]. Following two consecutive daily intraperitoneal injection of bovine serum albumin (BSA), kidney…
Fig. 38.1 A flow chart showing a series of conventional preparation procedures (a), freezing (b), in vivo cryotechnique (c), and cryobiopsy (d), followed by freeze-substitution (e) and immunostaining (f) or…
Fig. 39.1 Serial sections of mouse cerebellum prepared with the conventional fixation-dehydration method and embedded in paraffin show pCREB immunoreactivity (a–d, f, g, i, j) and DAPI (e, g, h,…
Fig. 20.1 (a) A schematic drawing of “in vivo cryotechnique ” for mouse kidneys under acute hypertensive condition . The abdominal aorta is ligated by thin thread just below both…
Fig. 37.1 (a) A schematic drawing of “in vivo cryotechnique ” (IVCT) for living mouse sciatic nerves under various stretching conditions. A sciatic nerve of an anesthetized mouse is exposed,…
Fig. 17.1 Light micrographs of serial paraffin sections of living mouse thymic tissues prepared by IVCT, which are stained with H&E (a, b) or immunostained for albumin (c, d), IgG1…
© Springer Japan 2016Shinichi Ohno, Nobuhiko Ohno and Nobuo Terada (eds.)In Vivo Cryotechnique in Biomedical Research and Application for Bioimaging of Living Animal Organs10.1007/978-4-431-55723-4_54 54. Concluding Remarks Shinichi Ohno1 , Nobuhiko Ohno1 and Nobuo Terada1, 2 (1) Department of Anatomy…
Fig. 33.1 Electron micrographs of molecular layers (a, b) or Purkinje cell layers (c) in mouse cerebellar cortex prepared by the “in vivo cryotechnique ” without ischemia (IVCT; a, c)…
Fig. 53.1 (a) Chemical structure of adenosine triphosphate (ATP) . (b, c) Schematic representation of the chemical attachment of ATP to a glass (b) or tissues (c) by the glutaraldehyde…
Fig. 29.1 Serial paraffin sections of antral follicles in the control (N; a–d, i–k, o–q, u–w) or mifepristone -induced polycystic ovary model (PCO; e–h, l−n, r–t, x–z) mouse ovaries prepared…
