Fig. 32.1 (a) A schematic representation of “in vivo cryotechnique ” (IVCT) for the mouse cerebellum after opening the scull; cryoknife is push into the cerebellum and simultaneously isopentane –propane…
Fig. 30.1 (a–d) Light micrographs of HE-staining (a, c) and immunostaining for albumin (b, d) in seminiferous tubules of the normal (a, b) and the 24 h cadmium -treated (c,…
Fig. 27.1 (a, b) Representative electron micrographs of the apical cytoplasm of umbrella cells in mouse bladder under the absence (a; Normal) and presence (b; Dynasore) of the dynasore treatment….
Fig. 10.1 Light micrographs of hematoxylin-eosin (HE) staining for paraffin sections of mesenteric lymph nodes prepared by IVCT followed by FS fixation. (a) Lower magnified view of a lymph node…
Fig. 8.1 Light micrographs of HE staining (HE; a, b), immunostaining for IgA (c), Igκ (d), IgG1 (e), and IgM (f), confocal laser scanning micrographs of double-fluorescence immunostaining for IgA…
Fig. 6.1 Three-dimensional reconstruction of triple-fluorescence images obtained by confocal laser scanning microscopy with PAS staining (red), immunostaining for immunoglobulin kappa light chain (green), and TO-PR-O3 (blue) in fasting mouse…
Fig. 12.1 Scanning electron micrographs of flowing erythrocyte s in abdominal aorta (a) and inferior vena cava (c) of living mice, in addition to both images reconstructed by serial ultrathin…
Fig. 16.1 Light (a) and electron (b–d) micrographs of pleural and pulmonary structures of inflating lungs of living mice, as prepared by the “in vivo cryotechnique ”. In the light…
Fig. 5.1 Sections prepared by various preparation methods and stained with hematoxylin-eosin show that widely open sinusoids are clearly detected with “in vivo cryotechnique ” under normal blood circulation (IVCT-N;…
Fig. 3.1 Simple manual IVCT for light microscopy by pouring the isopentane -propane (IP) cryogen over targeted organs of anesthetized animals 3.4 How to Prepare the Mixed Isopentane-Propane Cryogen Step…
