Fig. 42.1 (a) Photographs of different sizes of quantum dot s (QDs; 4.5, 6, and 7 nm) emitting different fluorescent colors under ultraviolet light . (b) Chemical structure of GSH-coated…
Fig. 41.1 (a–c) Fluorescence micrographs of quick-frozen, freeze-substituted, and freeze-dried TRITC- and FITC-conjugated goat immunoglobulins, which were mixed on glass slide from different direction (arrows in a, b). (c) is…
Fig. 40.1 Replica electron micrographs of intestinal smooth muscle cell s in living mice prepared by the IVCT followed by replica preparation method. (a) Freeze-fractured cytoplasm of three parallel-lining smooth…
Fig. 22.1 Representative light micrograph (a) and confocal laser scanning micrographs (b–d) showing periodic acid-Schiff staining ( PAS ; a), its fluorescence emission ( PAS fluorescence emission; b), and immunofluorescence…
Fig. 11.1 Light micrographs of hematoxylin–eosin (HE)-stained sections of normal mouse spleen , prepared with the in vivo cryotechnique (IVCT) followed by freeze-substitution (FS). (a) Low-magnified view of the white…
Fig. 36.1 (a–f) Differential interference contrast (DIC; a, c, e) and the glutamate immunostaining (Glu; b, d, f) images of the eyeball tissue sections with IVCT followed by FS with…
Fig. 9.1 Light microscopic images of HE staining (a, d, g, j, m, p, s, v) and immunostaining of ERK1/2 (b, e, h, k, n, q, t, w) and phosphorylated…
Fig. 19.1 Light micrographs of mouse renal cortical tissues prepared under various hemodynamic condition s ; “in vivo cryotechnique ” under normotensive (a, normal), heart-arrest (b, heart-arrest), and acute hypertensive…
Fig. 35.1 (a) Flow chart of procedure with IVCT and FS for immunohistochemical detection of phosphorylated protein. Dynamically changing molecule under phosphorylation is immediately immobilized with IVCT. (b) Schematic representation…
© Springer Japan 2016Shinichi Ohno, Nobuhiko Ohno and Nobuo Terada (eds.)In Vivo Cryotechnique in Biomedical Research and Application for Bioimaging of Living Animal Organs10.1007/978-4-431-55723-4_34 34. Overview on Recent Applications of In Vivo Cryotechnique in Neurosciences Nobuhiko Ohno1 ,…
