Advantages Limitations 1. Reduction of ischemic/anoxic artifacts by direct cryofixation 1. Preferred exposure of target organs for increasing regions without visible ice crystals 2. High time resolution by immediate freezing…
Fig. 44.1 (a) Raman spectra of human whole blood at low temperature (−150 °C) under different SO2 conditions. (b) Schematic representation of Raman cryomicroscopy for living mouse intestines, prepared with…
Fig. 18.1 Light micrographs of hematoxylin-eosin staining of paraffin sections of living mouse thymic tissues prepared by IVCT (a–f). (a) A low-magnification view of the thymic tissues shows widely panoramic…
Fig. 43.1 (a) Schematic representation of Raman microscopic analysis for the freeze-dried samples (arrow) sandwiched between glasses. (b, c) Raman mapping image (b) and morphological image with toluidine-blue staining (c)…
Fig. 28.1 Serial paraffin sections prepared by in vivo cryotechnique and stained with hematoxylin-eosin (HE, a) or immunostained for albumin (Alb, b), immunoglobulin G 1 (IgG1 , c), or IgM…
Fig. 42.1 (a) Photographs of different sizes of quantum dot s (QDs; 4.5, 6, and 7 nm) emitting different fluorescent colors under ultraviolet light . (b) Chemical structure of GSH-coated…
Fig. 40.1 Replica electron micrographs of intestinal smooth muscle cell s in living mice prepared by the IVCT followed by replica preparation method. (a) Freeze-fractured cytoplasm of three parallel-lining smooth…
Fig. 41.1 (a–c) Fluorescence micrographs of quick-frozen, freeze-substituted, and freeze-dried TRITC- and FITC-conjugated goat immunoglobulins, which were mixed on glass slide from different direction (arrows in a, b). (c) is…
Fig. 22.1 Representative light micrograph (a) and confocal laser scanning micrographs (b–d) showing periodic acid-Schiff staining ( PAS ; a), its fluorescence emission ( PAS fluorescence emission; b), and immunofluorescence…
Fig. 11.1 Light micrographs of hematoxylin–eosin (HE)-stained sections of normal mouse spleen , prepared with the in vivo cryotechnique (IVCT) followed by freeze-substitution (FS). (a) Low-magnified view of the white…
