Fig. 20.1 (a) A schematic drawing of “in vivo cryotechnique ” for mouse kidneys under acute hypertensive condition . The abdominal aorta is ligated by thin thread just below both…
Fig. 37.1 (a) A schematic drawing of “in vivo cryotechnique ” (IVCT) for living mouse sciatic nerves under various stretching conditions. A sciatic nerve of an anesthetized mouse is exposed,…
Fig. 17.1 Light micrographs of serial paraffin sections of living mouse thymic tissues prepared by IVCT, which are stained with H&E (a, b) or immunostained for albumin (c, d), IgG1…
© Springer Japan 2016Shinichi Ohno, Nobuhiko Ohno and Nobuo Terada (eds.)In Vivo Cryotechnique in Biomedical Research and Application for Bioimaging of Living Animal Organs10.1007/978-4-431-55723-4_54 54. Concluding Remarks Shinichi Ohno1 , Nobuhiko Ohno1 and Nobuo Terada1, 2 (1) Department of Anatomy…
Fig. 33.1 Electron micrographs of molecular layers (a, b) or Purkinje cell layers (c) in mouse cerebellar cortex prepared by the “in vivo cryotechnique ” without ischemia (IVCT; a, c)…
Fig. 53.1 (a) Chemical structure of adenosine triphosphate (ATP) . (b, c) Schematic representation of the chemical attachment of ATP to a glass (b) or tissues (c) by the glutaraldehyde…
Fig. 29.1 Serial paraffin sections of antral follicles in the control (N; a–d, i–k, o–q, u–w) or mifepristone -induced polycystic ovary model (PCO; e–h, l−n, r–t, x–z) mouse ovaries prepared…
Advantages Limitations 1. Reduction of ischemic/anoxic artifacts by direct cryofixation 1. Preferred exposure of target organs for increasing regions without visible ice crystals 2. High time resolution by immediate freezing…
Fig. 44.1 (a) Raman spectra of human whole blood at low temperature (−150 °C) under different SO2 conditions. (b) Schematic representation of Raman cryomicroscopy for living mouse intestines, prepared with…
Fig. 18.1 Light micrographs of hematoxylin-eosin staining of paraffin sections of living mouse thymic tissues prepared by IVCT (a–f). (a) A low-magnification view of the thymic tissues shows widely panoramic…
