Fig. 35.1 (a) Flow chart of procedure with IVCT and FS for immunohistochemical detection of phosphorylated protein. Dynamically changing molecule under phosphorylation is immediately immobilized with IVCT. (b) Schematic representation…
© Springer Japan 2016Shinichi Ohno, Nobuhiko Ohno and Nobuo Terada (eds.)In Vivo Cryotechnique in Biomedical Research and Application for Bioimaging of Living Animal Organs10.1007/978-4-431-55723-4_34 34. Overview on Recent Applications of In Vivo Cryotechnique in Neurosciences Nobuhiko Ohno1 ,…
Fig. 15.1 Morphological features of mouse lungs. (a, c) Macroscopic images of normal mouse lungs under experimental expiration (Ex) and inspiration (In). (b, d) Light microscopic images with HE staining…
Fig. 32.1 (a) A schematic representation of “in vivo cryotechnique ” (IVCT) for the mouse cerebellum after opening the scull; cryoknife is push into the cerebellum and simultaneously isopentane –propane…
Fig. 30.1 (a–d) Light micrographs of HE-staining (a, c) and immunostaining for albumin (b, d) in seminiferous tubules of the normal (a, b) and the 24 h cadmium -treated (c,…
Fig. 27.1 (a, b) Representative electron micrographs of the apical cytoplasm of umbrella cells in mouse bladder under the absence (a; Normal) and presence (b; Dynasore) of the dynasore treatment….
Fig. 10.1 Light micrographs of hematoxylin-eosin (HE) staining for paraffin sections of mesenteric lymph nodes prepared by IVCT followed by FS fixation. (a) Lower magnified view of a lymph node…
Fig. 8.1 Light micrographs of HE staining (HE; a, b), immunostaining for IgA (c), Igκ (d), IgG1 (e), and IgM (f), confocal laser scanning micrographs of double-fluorescence immunostaining for IgA…
Fig. 6.1 Three-dimensional reconstruction of triple-fluorescence images obtained by confocal laser scanning microscopy with PAS staining (red), immunostaining for immunoglobulin kappa light chain (green), and TO-PR-O3 (blue) in fasting mouse…
Fig. 12.1 Scanning electron micrographs of flowing erythrocyte s in abdominal aorta (a) and inferior vena cava (c) of living mice, in addition to both images reconstructed by serial ultrathin…
