Fig. 8.1 Light micrographs of HE staining (HE; a, b), immunostaining for IgA (c), Igκ (d), IgG1 (e), and IgM (f), confocal laser scanning micrographs of double-fluorescence immunostaining for IgA…
Fig. 10.1 Light micrographs of hematoxylin-eosin (HE) staining for paraffin sections of mesenteric lymph nodes prepared by IVCT followed by FS fixation. (a) Lower magnified view of a lymph node…
Fig. 12.1 Scanning electron micrographs of flowing erythrocyte s in abdominal aorta (a) and inferior vena cava (c) of living mice, in addition to both images reconstructed by serial ultrathin…
Fig. 6.1 Three-dimensional reconstruction of triple-fluorescence images obtained by confocal laser scanning microscopy with PAS staining (red), immunostaining for immunoglobulin kappa light chain (green), and TO-PR-O3 (blue) in fasting mouse…
Fig. 16.1 Light (a) and electron (b–d) micrographs of pleural and pulmonary structures of inflating lungs of living mice, as prepared by the “in vivo cryotechnique ”. In the light…
Fig. 13.1 Electron micrographs of intestinal smooth muscle cell s in normal scn mice (a, b) and dystrophin-deficient mdx mice (c, d). They were prepared by the “in vivo cryotechnique…
Fig. 3.1 Simple manual IVCT for light microscopy by pouring the isopentane -propane (IP) cryogen over targeted organs of anesthetized animals 3.4 How to Prepare the Mixed Isopentane-Propane Cryogen Step…
Fig. 5.1 Sections prepared by various preparation methods and stained with hematoxylin-eosin show that widely open sinusoids are clearly detected with “in vivo cryotechnique ” under normal blood circulation (IVCT-N;…
Fig. 7.1 (a) Pimonidazole binds to general proteins under ischemia due to adduction, and the antibody reacts to the protein adduct s of reductively activated pimonidazole (PARaPi). (b) Experimental protocol…
Fig. 2.1 Photographs of the newly developed “in vivo cryoapparatu s .” (a) The main part of the apparatus set up on a table. (b) Another system operating the valves…
