Inhalation Anesthesia in South American Camelids
Chapter 47 Inhalation Anesthesia in South American Camelids Thomas Riebold Increased interest in llamas, and more recently in alpacas, as pets and as breeding and pack animals has led to…
Embryo Preservation and in Vitro Production of Embryos
Chapter 29 Embryo Preservation and in Vitro Production of Embryos Virginia L. Trasorras, Susana M. Giuliano and Marcelo H. Miragaya Reproductive biotechnology techniques, which include artificial insemination with cooled or…
Camelid Vaccinations
Chapter 3 Camelid Vaccinations Patrick O. Long Vaccination protocols for camelids vary widely in the United States. Variations occur even regionally. Establishing a rational immunization protocol for clients can be…
Biosecurity
Chapter 1 Biosecurity George M. Barrington Biosecurity involves efforts designed to prevent introduction and spread of disease within populations, herds, or groups of animals.1 These efforts may be further divided…
Proteomics Characterization of Tick-Host-Pathogen Interactions
Database search Buffer 1 Buffer 2 Buffer 3 Buffer 4 Ixodida 62.9 % 48.2 % 44.4 % 82.0 % Pecora 72.4 % 44.2 % 40.2 % 72.7 % aThe efficacy…
Fluorescence In Situ Hybridization for the Tissue Detection of Bacterial Pathogens Associated with Porcine Infections
Target species Tissue sample Systematic namea Probe sequence (5′–3′) References Actinobacillus pleuropneumoniae Lung, ear, joints, bone, brain S-S-Ap-0185-a-A-19 CCCACCCTTTAATCCATAG [11–14] Genus Brachyspira Intestine L-G-Serp-1410-a-A-19 GTCATTCCATCGAAACATA [15–17] Brachyspira hyodysenteriae Intestine L-S-S.hyo-1210-a-A-19…
Pulsed-Field Gel Electrophoresis (PFGE): Application in Population Structure Studies of Bovine Mastitis-Causing Streptococci
Fig. 1 Schematic drawing of DNA agarose discs preparation (adapted from Chung et al. [11]). Source: H. de Lencastre, ITQB. Portugal) 5. Pipette 150 μl of the OD620 = 5.0 cell…
Next-Generation Sequencing in Veterinary Medicine: How Can the Massive Amount of Information Arising from High-Throughput Technologies Improve Diagnosis, Control, and Management of Infectious Diseases?
Fig. 1 Steps in next-generation sequencing and data analysis workflows where error and bias introduction may occur Fig. 2 High-throughput technologies can be applied to numerous aspects of animal infectious…
Detection of Fish Pathogens by Loop-Mediated Isothermal Amplification (LAMP) Technique
Target virus Primer sequences (5′–3′) References VHSa F3—GGS AAG CAA GGA YCA CGA G [13] B3—CAG GTG TCC YTC TAG TGT TTC FIP—GAT CCA CCG ATA CTG TTT TTG GGG…
Biological Specimen Collection and Processing for Molecular Analysis
Clinical signs Sample to submit for testing Hepatitis Liver Drop in egg production Feces/large intestines, vaginal swabs Lameness Synovial fluid, tendons, heart, liver Respiratory Tracheas, tracheal swabs, feces, cloacal swabs,…
