Tandem Stenosis to Induce Atherosclerotic Plaque Instability in the Mouse
Fig. 1 Schematic drawing of the tandem stenosis. ApoE−/− mice at 6–8 weeks of age are fed with Western diet containing 22 % fat and 0.15 % cholesterol and tap…
Bone Marrow Transplantation in Mice to Study the Role of Hematopoietic Cells in Atherosclerosis
Fig. 1 Illustrative example of bone marrow transplantation to generate mixed chimeras in mice. Six- to eight-week-old recipient mice are irradiated with myeloablative doses of radiation. In this example, femurs…
Analysis of Gene and Protein Expression in Atherosclerotic Mouse Aorta by Western Blot and Quantitative Real-Time PCR
Resolving gel (%) 5 7.5 10 12 15 Total vol 8 ml 8 ml 8 ml 8 ml 8 ml ddH2O 4.5 ml 3.8 ml 3.2 ml 2.6 ml 1.8…
Cytokines and Immune Responses in Murine Atherosclerosis
Fig. 1 The primary cytokines involved in atherosclerosis. In red: atherogenic cytokines. In green: atheroprotective cytokines. In blue: exact role still under investigation Fig. 2 Cytokines in the immune response…
Intravital Microscopy for Atherosclerosis Research
Fig. 1 Overview of image modalities for intravital microscopy. (a) comparison of characteristic image properties (i.e., axial and lateral resolution, acquisition speed, depth penetration multichannel detection, and motion artifact vulnerability)…
Noninvasive Molecular Imaging of Mouse Atherosclerosis
Fig. 1 Treatment with minocycline and ebselen reduced endothelial permeability, plaque burden, and gadofosveset uptake. A1 through D1 and A2 through D2, Cross-sectional delayed enhancement magnetic resonance imaging (DE-MRI) fused…
Isolation of Mouse Primary Aortic Endothelial Cells by Selection with Specific Antibodies
Fig. 1 Steps in mouse aortic endothelial cell extraction and culture. (a) Dissection of the animal to expose the thoracic and abdominal cavities. (b) Isolation of the aorta and heart…
Use of Mouse Models in Atherosclerosis Research
Advantages Atherosclerosis develops over a period of months Relative ease of genetic manipulation to allow for investigation of multiple cell types or proteins, including temporal or cell specific expression, on…
Isolation, Culture, and Polarization of Murine Bone Marrow-Derived and Peritoneal Macrophages
© Springer Science+Business Media New York 2015Vicente Andrés and Beatriz Dorado (eds.)Methods in Mouse AtherosclerosisMethods in Molecular Biology133910.1007/978-1-4939-2929-0_6 6. Isolation, Culture, and Polarization of Murine Bone Marrow-Derived and Peritoneal Macrophages Inés Pineda-Torra1 , Matthew Gage1, Alba de Juan2 and Oscar M. Pello1,…
