Fig. 1 Schematic drawing of the pressure myograph setup used for the described protocols 1. Regular microscope. 2. Micro-forceps, micro-scissors, and petri dish for dissection. 3. Glass pipettes…
Fig. 1 Schematic describing aortic flow cytometry protocol 2 Materials 2.1 Harvest Aorta 1. Microdissection tools. 2. Surgical microscope (e.g., Diagnostic Instruments Inc., Sterling Heights, Michigan). 3. 1×…
Fig. 1 Tools to dissect and mount the vessels into the wire myograph. The small petri dish contains 2-cm-long wires. The blue mark on the petri dish marks a length…
Fig. 1 PET/CT scanner and different equipment needed for anesthesia, injection of CT contrast agent, and temperature control (a) CT subsystem (see Note 3 ). (b) PET subsystem (see…
Fig. 1 Mouse board for cremaster muscle intravital microscopy. The board should be maintained in a constant temperature (36.5–37.5 °C) with a heating system. There are two areas in the…
Fig. 1 Gravity flow apparatus for vascular perfusion. Perfusant is suspended at a height of 60 cm above the mice to be perfused so as to maintain a pressure of…
Fig. 1 Morphology of normal and apoptotic smooth muscle cell nuclei. (a) Control mouse carotid artery demonstrating normal nuclear morphology. (b) Carotid artery showing nuclear condensation (filled arrows) compared to…
Fig. 1 Schematic diagram of the biliary macrophage-RCT pathway. Cholesteryl esters (CE) stored in lipid droplets of macrophage foam cells are hydrolyzed by neutral cholesteryl ester hydrolases. Lipid-poor nascent preβ-migrating…
Fig. 1 Double-collagenase digestion procedure for VSMC isolation from aorta 3.2 Mouse Aorta Dissection 1. Select mice from which aortas will be dissected. Usually 5–10 mice are required for…
Fig. 1 Immunomagnetic selection of cells. (1) The biotinylated antibodies are bound to the positive cells. (2) The streptavidin-microbeads bind to the biotinylated antibodies. (3) The magnetic columns are placed…
