Application of “In Vivo Cryotechnique” to Immunohistochemical Detection of Hypoxia in Mouse Liver Tissues Treated with Pimonidazole
Fig. 7.1 (a) Pimonidazole binds to general proteins under ischemia due to adduction, and the antibody reacts to the protein adduct s of reductively activated pimonidazole (PARaPi). (b) Experimental protocol…