Application of “In Vivo Cryotechnique” to Immunohistochemical Detection of Hypoxia in Mouse Liver Tissues Treated with Pimonidazole

Oct 9, 2016 by in GENERAL Comments Off on Application of “In Vivo Cryotechnique” to Immunohistochemical Detection of Hypoxia in Mouse Liver Tissues Treated with Pimonidazole

Fig. 7.1 (a) Pimonidazole binds to general proteins under ischemia due to adduction, and the antibody reacts to the protein adduct s of reductively activated pimonidazole (PARaPi). (b) Experimental protocol…

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Technical Merits with “IVCT”

Oct 9, 2016 by in GENERAL Comments Off on Technical Merits with “IVCT”

© Springer Japan 2016Shinichi Ohno, Nobuhiko Ohno and Nobuo Terada (eds.)In Vivo Cryotechnique in Biomedical Research and Application for Bioimaging of Living Animal Organs10.1007/978-4-431-55723-4_4 4. Technical Merits with “IVCT” Shinichi Ohno1   (1) Department of Anatomy and Molecular…

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Introduction

Oct 9, 2016 by in GENERAL Comments Off on Introduction

Fig. 1.1 A flowchart of various preparation steps for light microscopy (LM), fluorescence microscopy (FM), transmission electron microscopy (TEM), and scanning electron microscopy (SEM). The perfusion – (a) or immersion-…

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