Target antigen Host species (label) Clone Dilution Comment Mouse CD3 Hamster 500A2 1:100 Permeabilization step required Mouse CD4 Rat H129.19 1:100 Mouse CD8 Rat 53-6.7 1:100 Mouse FoxP3 Rat FJK-16s…
Fig. 1 Work flow of the phagocytosis assay with estimated times for each step. MΦ, macrophages; RBCs, red blood cells; C3, complement component 3 2 Materials 2.1 Establishing Macrophage Primary…
Fig. 1 Representative image of Ki-67 immunohistochemical staining in mouse early-stage atherosclerotic plaques. apoE-KO mice were fed a high-fat/high-cholesterol diet for 6 weeks. 6 μm-thick histological sections were obtained from…
Fig. 1 Atherosclerotic arteries harbor distinct immune cell infiltrates in plaques and adventitia. The adventitia of the normal arterial wall (left) constitutively contains vasa vasorum, lymph vessels, tissue macrophages, few…
Mouse number BW (g) Glucose solution volume (μl) to inject Glucose levels (mg/dL) 0 min 15 min 30 min 60 min 120 min 1 2 3 4 5 6 …
Fig. 1 Dissection tools 8. 10 ml syringes. 9. 1.5 ml Eppendorf tubes. 10. Phosphate-buffered saline (PBS): 1.54 mM KH2PO4, 155.17 mM NaCl, 2.70 mM Na2HPO4, pH…
, where [Fluo] i and [Exc] i represent the fluorescence and excitation image for source position i, respectively, and s is a smoothing factor that prevents division by very small…
Fig. 1 Surgical preparation and stabilization of the mouse carotid artery. Numbers indicate the different elements and tissues involved in the procedure. (a) Mice are immobilized in decubitus position, the…
Fig. 1 Schematic drawing of the tandem stenosis. ApoE−/− mice at 6–8 weeks of age are fed with Western diet containing 22 % fat and 0.15 % cholesterol and tap…
Fig. 1 Illustrative example of bone marrow transplantation to generate mixed chimeras in mice. Six- to eight-week-old recipient mice are irradiated with myeloablative doses of radiation. In this example, femurs…
