Fig. 1 Immunomagnetic selection of cells. (1) The biotinylated antibodies are bound to the positive cells. (2) The streptavidin-microbeads bind to the biotinylated antibodies. (3) The magnetic columns are placed…
Fig. 1 Double-collagenase digestion procedure for VSMC isolation from aorta 3.2 Mouse Aorta Dissection 1. Select mice from which aortas will be dissected. Usually 5–10 mice are required for…
Fig. 1 Representative T1-weighted MR serial images showing the abdominal aorta (white arrow) above the right renal artery of an apoE-KO mouse demonstrating large atherosclerotic lesions protruding the arterial lumen….
Intervention [reference] Agea (weeks) Diet Brachiocephalic artery (%) Carotid artery (%) None [3, 4] 20 WTD 8 0 Age [5] 42–50 Chow 66–75 n.d. Collar [7] 52 WTD n.a. 8…
Target antigen Host species (label) Clone Dilution Comment Mouse CD3 Hamster 500A2 1:100 Permeabilization step required Mouse CD4 Rat H129.19 1:100 Mouse CD8 Rat 53-6.7 1:100 Mouse FoxP3 Rat FJK-16s…
Fig. 1 Work flow of the phagocytosis assay with estimated times for each step. MΦ, macrophages; RBCs, red blood cells; C3, complement component 3 2 Materials 2.1 Establishing Macrophage Primary…
Fig. 1 Representative image of Ki-67 immunohistochemical staining in mouse early-stage atherosclerotic plaques. apoE-KO mice were fed a high-fat/high-cholesterol diet for 6 weeks. 6 μm-thick histological sections were obtained from…
Fig. 1 Atherosclerotic arteries harbor distinct immune cell infiltrates in plaques and adventitia. The adventitia of the normal arterial wall (left) constitutively contains vasa vasorum, lymph vessels, tissue macrophages, few…
Mouse number BW (g) Glucose solution volume (μl) to inject Glucose levels (mg/dL) 0 min 15 min 30 min 60 min 120 min 1 2 3 4 5 6 …
Fig. 1 Dissection tools 8. 10 ml syringes. 9. 1.5 ml Eppendorf tubes. 10. Phosphate-buffered saline (PBS): 1.54 mM KH2PO4, 155.17 mM NaCl, 2.70 mM Na2HPO4, pH…
