Fig. 1 Gravity flow apparatus for vascular perfusion. Perfusant is suspended at a height of 60 cm above the mice to be perfused so as to maintain a pressure of…
Fig. 1 Morphology of normal and apoptotic smooth muscle cell nuclei. (a) Control mouse carotid artery demonstrating normal nuclear morphology. (b) Carotid artery showing nuclear condensation (filled arrows) compared to…
Fig. 1 Schematic diagram of the biliary macrophage-RCT pathway. Cholesteryl esters (CE) stored in lipid droplets of macrophage foam cells are hydrolyzed by neutral cholesteryl ester hydrolases. Lipid-poor nascent preβ-migrating…
Fig. 1 Immunomagnetic selection of cells. (1) The biotinylated antibodies are bound to the positive cells. (2) The streptavidin-microbeads bind to the biotinylated antibodies. (3) The magnetic columns are placed…
Fig. 1 Double-collagenase digestion procedure for VSMC isolation from aorta 3.2 Mouse Aorta Dissection 1. Select mice from which aortas will be dissected. Usually 5–10 mice are required for…
Fig. 1 Representative T1-weighted MR serial images showing the abdominal aorta (white arrow) above the right renal artery of an apoE-KO mouse demonstrating large atherosclerotic lesions protruding the arterial lumen….
Intervention [reference] Agea (weeks) Diet Brachiocephalic artery (%) Carotid artery (%) None [3, 4] 20 WTD 8 0 Age [5] 42–50 Chow 66–75 n.d. Collar [7] 52 WTD n.a. 8…
Target antigen Host species (label) Clone Dilution Comment Mouse CD3 Hamster 500A2 1:100 Permeabilization step required Mouse CD4 Rat H129.19 1:100 Mouse CD8 Rat 53-6.7 1:100 Mouse FoxP3 Rat FJK-16s…
Fig. 1 Work flow of the phagocytosis assay with estimated times for each step. MΦ, macrophages; RBCs, red blood cells; C3, complement component 3 2 Materials 2.1 Establishing Macrophage Primary…
Fig. 1 Representative image of Ki-67 immunohistochemical staining in mouse early-stage atherosclerotic plaques. apoE-KO mice were fed a high-fat/high-cholesterol diet for 6 weeks. 6 μm-thick histological sections were obtained from…
