Target pathogen(s) POC Amplification method LOD or sensitivity References Yes No African swine fever virus × LAMP ≤330 copies [67] Avian influenza virus (H5N1) × RT-PCR – [10] Avian influenza…
Fig. 1 Schematic representation of the RLB hybridization process. (a) Oligoprobes are covalently coupled to the membrane in lines using a line-blotter apparatus; (b) the membrane is then rotated 90°;…
Fig. 1 Mycobacterium bovis cells captured by monoclonal antibody and biotinylated 12-mer peptide-coated Dynabeads® MyOne™ Tosylactivated—a large clump of M. bovis cells and an individual cell are clearly seen attached…
Fig. 1 Step-by-step protocol for the molecular diagnosis of strongylid nematode infections from small ruminant fecal samples. Following coproscopy (1). the fecal suspension is washed and nematode parasite eggs are…
Oligo name Sequence (5′–3′) Working concentration (μM) Primer Hofm_BTV_IVI_F2 TGG AYA AAG CRA TGT CAA A 20 Primer Hofm_BTV_IVI_R2 ACR TCA TCA CGA AAC GCT TC 20 Probe Hofm_BTV_IVI_Pa FAM-ARG…
© Springer Science+Business Media New York 2015Mónica V. Cunha and João Inácio (eds.)Veterinary Infection Biology: Molecular Diagnostics and High-Throughput StrategiesMethods in Molecular BiologyMethods and Protocols124710.1007/978-1-4939-2004-4_7 7. Molecular Approaches to Recognize Relevant and Emerging Infectious Diseases…
© Springer Science+Business Media New York 2015Mónica V. Cunha and João Inácio (eds.)Veterinary Infection Biology: Molecular Diagnostics and High-Throughput StrategiesMethods in Molecular BiologyMethods and Protocols124710.1007/978-1-4939-2004-4_2 2. Significance and Integration of Molecular Diagnostics in the Framework…
Strengths Weaknesses • Overall higher analytical and diagnostic sensitivities and specificities, higher accuracy, and shorter time-to-result • Suitable for the analysis of a large number of samples (e.g., high sample…
