Fig. 16.1 Light (a) and electron (b–d) micrographs of pleural and pulmonary structures of inflating lungs of living mice, as prepared by the “in vivo cryotechnique ”. In the light…
Fig. 5.1 Sections prepared by various preparation methods and stained with hematoxylin-eosin show that widely open sinusoids are clearly detected with “in vivo cryotechnique ” under normal blood circulation (IVCT-N;…
Fig. 3.1 Simple manual IVCT for light microscopy by pouring the isopentane -propane (IP) cryogen over targeted organs of anesthetized animals 3.4 How to Prepare the Mixed Isopentane-Propane Cryogen Step…
Fig. 13.1 Electron micrographs of intestinal smooth muscle cell s in normal scn mice (a, b) and dystrophin-deficient mdx mice (c, d). They were prepared by the “in vivo cryotechnique…
Fig. 7.1 (a) Pimonidazole binds to general proteins under ischemia due to adduction, and the antibody reacts to the protein adduct s of reductively activated pimonidazole (PARaPi). (b) Experimental protocol…
Fig. 2.1 Photographs of the newly developed “in vivo cryoapparatu s .” (a) The main part of the apparatus set up on a table. (b) Another system operating the valves…
Fig. 1.1 A flowchart of various preparation steps for light microscopy (LM), fluorescence microscopy (FM), transmission electron microscopy (TEM), and scanning electron microscopy (SEM). The perfusion – (a) or immersion-…
© Springer Japan 2016Shinichi Ohno, Nobuhiko Ohno and Nobuo Terada (eds.)In Vivo Cryotechnique in Biomedical Research and Application for Bioimaging of Living Animal Organs10.1007/978-4-431-55723-4_4 4. Technical Merits with “IVCT” Shinichi Ohno1 (1) Department of Anatomy and Molecular…
Fig. 1.1 Title I of the FSMA concerns the improvement of the capability of preventing food safety problems by means of different actions: the inspection of records concerning ‘suspect’ foods; the…
© The Author(s) 2016Arpan Bhagat, Giorgia Caruso, Maria Micali and Salvatore ParisiFoods of Non-Animal OriginSpringerBriefs in Molecular Science10.1007/978-3-319-25649-8_3 3. Chemistry and Technology of Ready-to-Eat Vegetable Foods Giorgia Caruso1 and Salvatore Parisi2 (1) Palermo, Italy (2) Palermo, Italy …
