Glucose and Insulin Tolerance Tests in the Mouse
Mouse number BW (g) Glucose solution volume (μl) to inject Glucose levels (mg/dL) 0 min 15 min 30 min 60 min 120 min 1 2 3 4 5 6 …
Oil Red O and Hematoxylin and Eosin Staining for Quantification of Atherosclerosis Burden in Mouse Aorta and Aortic Root
Fig. 1 Dissection tools 8. 10 ml syringes. 9. 1.5 ml Eppendorf tubes. 10. Phosphate-buffered saline (PBS): 1.54 mM KH2PO4, 155.17 mM NaCl, 2.70 mM Na2HPO4, pH…
Fluorescent Molecular Tomography for In Vivo Imaging of Mouse Atherosclerosis
, where [Fluo] i and [Exc] i represent the fluorescence and excitation image for source position i, respectively, and s is a smoothing factor that prevents division by very small…
Mechanical Stabilization of Mouse Carotid Artery for In Vivo Intravital Microscopy Imaging of Atherogenesis
Fig. 1 Surgical preparation and stabilization of the mouse carotid artery. Numbers indicate the different elements and tissues involved in the procedure. (a) Mice are immobilized in decubitus position, the…
Tandem Stenosis to Induce Atherosclerotic Plaque Instability in the Mouse
Fig. 1 Schematic drawing of the tandem stenosis. ApoE−/− mice at 6–8 weeks of age are fed with Western diet containing 22 % fat and 0.15 % cholesterol and tap…
Bone Marrow Transplantation in Mice to Study the Role of Hematopoietic Cells in Atherosclerosis
Fig. 1 Illustrative example of bone marrow transplantation to generate mixed chimeras in mice. Six- to eight-week-old recipient mice are irradiated with myeloablative doses of radiation. In this example, femurs…
Analysis of Gene and Protein Expression in Atherosclerotic Mouse Aorta by Western Blot and Quantitative Real-Time PCR
Resolving gel (%) 5 7.5 10 12 15 Total vol 8 ml 8 ml 8 ml 8 ml 8 ml ddH2O 4.5 ml 3.8 ml 3.2 ml 2.6 ml 1.8…
Cytokines and Immune Responses in Murine Atherosclerosis
Fig. 1 The primary cytokines involved in atherosclerosis. In red: atherogenic cytokines. In green: atheroprotective cytokines. In blue: exact role still under investigation Fig. 2 Cytokines in the immune response…
Intravital Microscopy for Atherosclerosis Research
Fig. 1 Overview of image modalities for intravital microscopy. (a) comparison of characteristic image properties (i.e., axial and lateral resolution, acquisition speed, depth penetration multichannel detection, and motion artifact vulnerability)…
Noninvasive Molecular Imaging of Mouse Atherosclerosis
Fig. 1 Treatment with minocycline and ebselen reduced endothelial permeability, plaque burden, and gadofosveset uptake. A1 through D1 and A2 through D2, Cross-sectional delayed enhancement magnetic resonance imaging (DE-MRI) fused…
