Basics in Rat MHC Genomics
Fig. 4.1 RT1 genomics Using serological and histogenetic typing, several haplotypes (combination of alleles in RT1-A and RT1-B/D regions) were defined. The haplotype is marked with upper case letters (a,…
Basic Equipment for Microsurgical Experiment
Fig. 2.1 Basic set of instruments Medical scissors (a) and surgical tweezers (b) can serve for laparotomy preparation, a needle holder for abdomen closing (c) as well as a pair…
Immunosuppresive Drugs Commonly Used in Transplantation Models
Donor strain (heart) Recipient strain Tacrolimus dose (mg/kg) The mean graft survival (days) Administration of the drug References Brown-Norway RT1n Lewis RT1I 1 10–15 Oral Deuse et al. [13] Brown-Norway…
Recovery After Surgery
© Springer International Publishing Switzerland 2015Peter Girman, Jan Kriz and Peter Balaz (eds.)Rat Experimental Transplantation Surgery10.1007/978-3-319-14559-4_7 7. Recovery After Surgery Eva Fabryova1 and Jan Kriz2 (1) Laboratory of Pancreatic Islets, Institute for Clinical and Experimental Medicine, Prague, Czech…
Pressure Myography to Study the Function and Structure of Isolated Small Arteries
Fig. 1 Schematic drawing of the pressure myograph setup used for the described protocols 1. Regular microscope. 2. Micro-forceps, micro-scissors, and petri dish for dissection. 3. Glass pipettes…
Flow Cytometric Analysis of Immune Cells Within Murine Aorta
Fig. 1 Schematic describing aortic flow cytometry protocol 2 Materials 2.1 Harvest Aorta 1. Microdissection tools. 2. Surgical microscope (e.g., Diagnostic Instruments Inc., Sterling Heights, Michigan). 3. 1×…
Wire Myography to Study Vascular Tone and Vascular Structure of Isolated Mouse Arteries
Fig. 1 Tools to dissect and mount the vessels into the wire myograph. The small petri dish contains 2-cm-long wires. The blue mark on the petri dish marks a length…
Intravital Microscopy in the Cremaster Muscle Microcirculation for Endothelial Dysfunction Studies
Fig. 1 Mouse board for cremaster muscle intravital microscopy. The board should be maintained in a constant temperature (36.5–37.5 °C) with a heating system. There are two areas in the…
In Vivo 18F-FDG-PET Imaging in Mouse Atherosclerosis
Fig. 1 PET/CT scanner and different equipment needed for anesthesia, injection of CT contrast agent, and temperature control (a) CT subsystem (see Note 3 ). (b) PET subsystem (see…
Immunostaining of Macrophages, Endothelial Cells, and Smooth Muscle Cells in the Atherosclerotic Mouse Aorta
Fig. 1 Gravity flow apparatus for vascular perfusion. Perfusant is suspended at a height of 60 cm above the mice to be perfused so as to maintain a pressure of…
