Fig. 33.1
Detection of chloroplast gene (clor, a), soybean lectin gene (lec, a), 35S promoter (35S, b), and CP4 EPSPS gene (CP4, b) fragments in milk samples from goats fed conventional (a) or transgenic (b) soybean s.e. meal
33.4 Discussion
According to other authors (Einspanier et al. 2001; Phipps et al. 2003; Poms et al. 2003; Tudisco et al. 2010), it has been confirmed that the detection of specific gene fragments is more difficult than that of nonspecific fragments. In addition, our results confirm the possibility to detect transgenic fragments in milk samples from animals fed GM feed, as previously reported (Tudisco et al. 2010). Concerning food traceability, the influence of sample storage on the concentration as well as on the quality of DNA extracted has been demonstrated; indeed, both parameters were decreased in the pellet of somatic cells stored at −20°C.
References
Bottero MT, Civera T, Nucera D, Rosati S, Sacchi P, Turi RM (2003) A multiplex polymerase chain reaction for the identification of cows’, goats’ and sheep’s milk in dairy products. Int Dairy J 13:277–282CrossRef
Chowdhury EH, Mikami O, Nakajima Y, Kuribara H, Hino A, Suga K, Hanazumi M, Yomemochi C (2003) Detection of genetically modified maize DNA fragments in the intestinal contents of pigs fed StarLinkTM CBH351. Vet Hum Toxicol 45:95–96PubMed
EFSA (2008) Safety and nutritional assessment of GM plants and derived food and feed: the role of animal feeding trials. Food Chem Toxicol 46:S2–S70
Einspanier R, Klotz A, Kraft J, Aulrich K, Poser R, Schwägele F, Jahreis G, Flachowsky G (2001) The fate of forage plant DNA in farm animals: a collaborative case-study investigating cattle and chicken fed recombinant plant material. Eur Food Res Technol 212:129–134CrossRef
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