L. grayi was isolated from 8% of the samples, L. ivanovii from 3.5%, and both L. innocua and L. welshimeri from 2.3% of the samples. S. napoli, sensitive to all tested antibiotics, and S. typhimurium, resistant to streptomycin, ampicillin, chloramphenicol, and sulfonamide, were isolated, respectively, from two samples of ice fish (PG 15 and PG 21). For the first serotype, only genes invA and phoP were identified, while S. typhimurium possessed the fliC gene (Fig. 25.2), which identified the serotype, and the virulence factors examined. Escherichia adecarboxylata, E. hermannii, Enterobacter cloacae, and Citrobacter youngae, microorganisms widespread in the environment but not necessarily related to fecal contamination, were isolated from 19.0% of the frozen samples. Shewanella putrefaciens, a spoilage bacterium highly widespread in frozen and chilled fishery products, was isolated in PG H₂O 11.