Fig. 7.1
Electrophoresis of the 16S–23S rRNA intergenic region PCR products by an Agilent Bioanalyzer. L ladder, A RS-PCR genotype B profile as described by Fournier et al. (2008); Lanes 1–16: RS-PCR profiles of samples analyzed in this preliminary study; (N) number of samples with the same profile
Table 7.1
Virulence genes of the strains with RS-PCR profiles 1 and 2
Profile | No. of strains | Enterotoxins (No. of strains) | spa | coa | leukE | clfA | LukE–LukD | mecA |
|---|---|---|---|---|---|---|---|---|
1 | 4 | sec (3), sed (3), sej (3) | >250 | >640 | 4 | 4 | 4 | 0 |
2 | 7 | seg (1), sei (1) | >100 | >640 | 1 | 5 | 0 | 7 |
7.4 Conclusions
The results obtained from this study revealed that the RS-PCR genotyping that was previously tested in Switzerland herds can be used in Italy as a rapid test that allows for grouping of heterogeneous S. aureus strains into defined subtypes. According to Fournier et al. (2008), the presence of a spa gene with a fragment size greater than 250 bp and a specific combination of genes encoding enterotoxins and leukotoxins is associated with virulence and pathogenic properties of the strain. This preliminary study has demonstrated the presence of genotype B strains in northern Italian herds. Additionally, unlike strains belonging to the RS-PCR profile type, none of the genotype B strains isolated in this study was mecA positive. The association of infectivity with high-profile resistance to antibiotics would require greater action to control disease and a strategic plan for the treatment of positive animals.
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