Jan K. Shearer The Code of Federal Regulations considers the term “livestock” to include cattle, sheep, horses (discussed separately in Chapter 4), goats, and other domestic animals ordinarily raised or used on the farm. Turkeys and domesticated fowl are considered poultry and not livestock. People keep and raise livestock mainly for production purposes and/or companionship. Clients who want to raise and keep livestock may do so for small “hobby” farming rather than large‐scale production. By caring for the animals, they learn about their unique behaviors and personalities and develop strong bonds of attachment best characterized as a pet relationship (Figure 5.1). These more intimate connections make end‐of‐life (EOL) decisions naturally more difficult. Practitioners will be asked to euthanize companion livestock in a variety of settings including small farms, petting zoos, animal rescues, and sanctuaries. In most cases, the preferred location for conducting euthanasia is the farm of origin. In some cases, practitioners are equipped to handle animals presented for euthanasia in their clinic facilities. For animals that are nonambulatory, euthanasia must be conducted at the farm. Regardless of where the procedure is performed, practitioners need to be aware of carcass disposal options relative to burial, composting, or landfills based on local laws and ordinances. Production livestock are raised in agricultural settings for the purpose of producing commodities such as food including milk and meat, and fiber, such as leather, wool, and mohair. Farms are businesses that must generate a profit, and like any enterprise that is not profitable, it will not last. Dairy farmers have dairy cows because they like dairy cows, but unless they are able to make enough milk to pay for the feed and a barn to house them, they won’t be milking them very long. Therefore, the primary objectives in production settings are performance and profit. However, it’s a love for the animals and a lifestyle that, no matter how meager, drives them forward. Livestock producers understand from experience that disease can have catastrophic consequences ending in death or the need for euthanasia. As the saying goes, “If you have livestock, you will have dead stock.” Ranchers see their cattle as individuals readily identifying their unique physical characteristics and personality traits (Irvine and Ellis 2010). They care about their animals and will do whatever is necessary to minimize their suffering in the face of illness or injury. The key to preserving the welfare of livestock is the early detection of disease or injury, prompt treatment as necessary, and humane euthanasia for animals with serious or terminal diseases. Options for an animal compromised by disease or injury include further treatment, slaughter, or euthanasia. The choice to euthanize an animal is based on: Figure 5.1 Two boys presenting their livestock. Specific indications for euthanasia include the following: When a practitioner is presented with an animal in grave condition from injury or disease, further examination is needed to determine if additional treatment is warranted or if euthanasia may be necessary (Figure 5.2). If it is determined that euthanasia is best for the welfare of the animal, and the client agrees, the discussion turns to the method to be used. Some may be unfamiliar with methods of euthanasia acceptable in livestock. They may be uncomfortable with the use of a firearm and unfamiliar with captive bolts or be under the mistaken impression that these methods are less humane than euthanasia by the intravenous (IV) administration of a barbiturate. This is a teaching opportunity for the practitioner; an opportunity to affirm perceptions that are accurate and correct those which are not. Figure 5.2 Determining factors for euthanasia of livestock. Source: Robert Callan, DVM. The methods of euthanasia acceptable for use in livestock are (1) the administration of a lethal drug overdose; (2) the use of a firearm; and (3) the use of penetrating or nonpenetrating captive bold with adjunctive methods to assure death. Each method has advantages and disadvantages and practitioners will have their preferred methods. Because specific conditions will differ, practitioners should be prepared to apply the euthanasia method that best fits the circumstances. One of the largest determining factors for method selection will be what happens with the carcass following death. Euthanasia by the IV administration of a lethal drug is common in livestock species, although some key considerations need to be made before proceeding (AVMA 2020). It’s possible that a physical method may be the better choice. Large determining factors include drug cost and availability, the ability to handle the patients, and carcass disposal. The cost of euthanasia may be considerably more due to the price of drugs, particularly for larger livestock. There may also be additional costs associated with the placement of an IV indwelling catheter and the use of other technical aids. Some euthanasia drugs are registered as controlled substances (e.g. pentobarbital sodium), which may be harder to obtain and carry in large quantities. Livestock can also be difficult to handle at times making injections more challenging. However, perhaps the greatest complication encountered when euthanizing livestock using lethal injection is disposal of the carcass. Carcasses that contain pentobarbital pose significant risks to wildlife and the environment (Wells et al. 2020). Regardless of the carcass disposal method chosen, rendering, burial, composting, or landfills, residues of pentobarbital will persist. See Chapter 7 for more information on carcass handling. Euthanasia by gunshot and captive bolt are more common in livestock compared to smaller mammals and are highly effective, but they are aesthetically bothersome for some observers. This is particularly true for people unfamiliar with these methods, which includes clients of pet livestock, young people with 4H and FFA livestock projects, or in situations where these techniques are used on neonates and young stock. While this may be difficult to explain in terms that make sense to animal clients, practitioners have an obligation to be sure that people understand why the method is chosen. For clients and others who choose to observe, it is important to share with them what observations they should expect to see and then describe the events as they occur in real‐time. This will help reassure observers and instill confidence that the procedure is progressing as expected. A small to moderate amount of hemorrhage may be expected, and on occasion, brain matter may be extruded through the site of the bullet or captive bolt. Although unpleasant to see, the extrusion of brain tissue confirms a good shot. To determine if the gunshot or captive bolt method was effective practitioners must know what constitutes unconsciousness. The physical observable parameters are: (1) an immediate collapse to the ground; (2) the body and muscles become rigid, with a gradual relaxation over a period of 15–20 seconds; (3) there is an immediate and sustained cessation of rhythmic breathing; (4) the animal makes no attempt to right itself or rise; (5) there is an absence of vocalization; and (6) there are no palpebral or corneal reflexes. These are important visual observations for practitioners because if these are not observed following the shot, it may indicate that the animal is regaining consciousness and should be shot again. Positioning and restraint of livestock are important for successful euthanasia procedures. For large livestock, one should also consider the animal’s location at the time of euthanasia and how that relates to human safety and ease of removal or disposal of the carcass. For example, cattle should not be euthanized in a chute or narrow alleyway unless there is a way of opening the side to remove the carcass (Figure 5.3). Good restraint is an important prerequisite for safe and efficient euthanasia of livestock. This may be accomplished with chemical restraint, physical restraint, or a combination of both. The guiding principle is to provide sufficient restraint to minimize stress, discomfort, or pain associated with the procedure. In practical terms, this implies sufficient restraint to either perform a competent venipuncture or sufficiently stabilize the head for an accurate gunshot or captive bolt stun. Figure 5.3 A chute with the side open prior to euthanasia. The head is restrained with a halter for safer euthanasia by IV injection, gunshot, or captive bolt stunning. The head is pulled slightly to the animal’s left so that the animal will fall to the right side. Except for very docile or obtunded animals, some degree of physical restraint is likely to be necessary to stabilize an animal’s head and neck. For manageable adult cattle and calves, a halter may be all that is needed. The halter can be secured to any convenient, sturdy object or, in some instances, held by an assistant. Sometimes, using two halters and cross‐tying the animal will provide more restraint. In recumbent cattle, a single halter can be placed, pulling the head to the side and securing the lead around the rear leg and tied at the level of the stifle to provide stabilization of the head and neck. This will provide good restraint allowing for either lethal injection or euthanasia by physical method. The mechanics of euthanasia for smaller ruminants and camelids is generally the same as in cattle except that the animals are smaller and more easily restrained without a chute. The goal is to position the animal so that the head and neck are accessible. For severely ill, obtunded, or recumbent animals, only minimal physical restraint may be necessary. Even in alert and active animals, gentle physical restraint is often sufficient. A halter can also be used. A head catch or chute is rarely needed to restrain a sheep or goat for euthanasia. Euthanasia of swine can be more challenging than other livestock species due to the difficulty of proper restraint and positioning. In general, pigs do not accept physical restraint well. In severely debilitated or obtunded animals, this may not be an issue. While pig snares can be used, this often results in significant resistance and distress for the patient. Another alternative for smaller pigs is to suspend them in a sling. This may allow access to the cephalic vein for IV injection. However, because of the challenges with restraint and venipuncture, humane euthanasia performed by lethal injection may be best performed following heavy sedation or anesthesia to allow for a calm venipuncture or intracardiac (IC) injection. Otherwise, gunshot or captive bolt methods should be utilized. Sedation may be appropriate for all livestock species, particularly when simple physical restraint is not sufficient, or if the client specifically requests it. The primary reason for using sedation would be if the animal cannot be restrained sufficiently to allow for an effective euthanasia method. The practitioner will need to use their discretion and err on the side of caution. Even though many different species are considered livestock, most of the same supplies can be used among them. IV administration of drugs requires access to needles, syringes, catheters, extension lines, tape, and sometimes clippers to remove hair or fiber. A variety of syringe sizes are necessary to accommodate the size of animals, ranging from neonates to adults. As with all species, the bigger the animal, the bigger the syringe needed. Needle sizes range from 20 to 18 gauge × 1.5 in. for small ruminants or camelids, and up to 14 gauge × 2 in. for adult cattle. If an IV catheter must be placed, 18–16 gauge up to 3 in. is ideal for small ruminants or camelids. Larger livestock do well with 16–14 gauge × 3–5 in. catheters. Spinal needles or extra‐long 6 in. needles may be needed for intrathecal (ITh) injections. Gloves should be on hand as well as ample amounts of the drugs themselves to ensure death is achieved. These will be described in greater detail in Section 5.4. Considering the carcass disposal problems with lethal injection using pentobarbital, practitioners working with livestock must be able to euthanize animals using a firearm or penetrating captive bolts. For practitioners with ambulatory practices who plan to use a firearm for euthanasia, there are several things to consider. Some states require anyone traveling with a firearm to have a concealed carry permit. Assuming a practitioner carries a firearm on calls in their practice vehicle, is the firearm loaded or kept separately from the ammunition? Is the firearm secure at all times? Are the bullets or shotshells maintained in a moisture‐proof container? A .22‐caliber rifle with a solid point bullet directed to the correct anatomic site will render most livestock immediately unconscious with a single shot. Soft or hollow point .22‐caliber bullets may be used in calves, sheep, or goats and will reduce the risk of the bullet exiting the target. Soft or hollow point .22‐caliber bullets may not penetrate the skull of adult cattle and are not recommended. A .22‐caliber long rifle solid point (round nose) bullet is better suited for adult cattle and swine but may still lack the muzzle energy to penetrate some large cows, bulls, or boars. A bullet’s muzzle velocity and energy are greater when fired from a rifle compared to a handgun. The longer barrel of a rifle allows the propellant (gunpowder) to burn more completely, thus propelling the bullet through the barrel and maximizing its velocity as it leaves the muzzle. The shorter barrel of a handgun results in reduced muzzle velocity since some, if not much, of the pressure from the burning of propellants is dissipated into the air as the bullet exits the barrel. A .22 long rifle or .22 magnum cartridge is generally sufficient for cattle >24 months of age, but some practitioners still prefer higher caliber firearms. The problem with higher caliber firearms is that the higher muzzle velocity and energy risks perforation (bullet exits the skull) of the skull by the bullet. For the reasons of safety, a bullet that penetrates the target but does not exit is preferred. For mature bulls and boars, a .357‐caliber or larger firearm is recommended. Hollow or soft point bullets may be considered to minimize the risk of the projectile exiting the animal and possibly causing human injury or property damage. This is particularly a concern when using a large caliber firearm for small ruminants or calves. Shotguns ranging from 20 to 12 gauges are recommended for larger livestock such as bulls or boars. Recommended firearms and cartridges for the euthanasia of livestock are listed in Table 5.1. Table 5.1 Recommended firearm and cartridge for euthanasia of livestock. Source: Adapted from Welfare aspects of animal stunning and killing methods (2004) European Food Safety Authority AHAW/04‐027. Scientific report of the Scientific Panel for Animal Health and Welfare on a request from the commission related to welfare aspects of animal stunning and killing methods. Although restrictions or laws on carrying a captive bolt are unlikely to be as strict, the issues relative to protecting the captive bolt gun and charges from moisture are similar to firearms. The most common cause of captive bolt malfunction is the failure to clean the gun after each use. Powder residue quickly draws moisture when the gun is not regularly cleaned and stored in a moisture‐free environment. The accumulation of powder residue and moisture leads to the development of rust, which, at worst, causes the pitting of the metal and malfunction of the captive bolt. Moisture also affects the powder charges used to drive the bolt; the result is soft shots (i.e. reduced bolt velocity). Both penetrating and nonpenetrating captive bolt stunners are available for the euthanasia of livestock. The nonpenetrating captive bolt device has a mushroom head and stuns the animal solely by concussive force. In general, the nonpenetrating captive bolt stunner is not considered a reliable method for the field euthanasia of adult livestock. Along with concussive impact to the skull, when properly placed, penetrating captive bolt stunners cause irreversible physical trauma to the brain, including portions of the cerebrum, thalamus, midbrain, pons, cerebellum, and medulla oblongata. Penetration and physical trauma to these areas of the brain will cause irreversible loss of consciousness provided the appropriate areas are damaged. Captive bolt guns are a safer option for routine on‐farm use. For this reason, the penetrating captive bolt stunner is generally preferred for veterinary euthanasia using physical methods. Pre‐euthanasia sedation is typically used in livestock in order to aid in restraint and improve safety for the practitioner, client, and other bystanders. In some cases of pet livestock, the client may request sedation prior to euthanasia. In many cases, only mild sedation is needed to allow for successful venipuncture, catheter placement, or accurate gunshot or stunning. With more excitable animals, deep sedation may be required to ensure that the animal remains recumbent for the procedure in order to provide adequate restraint and safety. Sedative drugs are relatively inexpensive and easy to obtain. Alpha‐2 adrenergic receptor agonists like xylazine are probably the most common sedatives used for livestock. Xylazine provides potent sedation along with analgesia at very low doses in ruminants and camelids. Acepromazine can also be used but will not provide additional analgesia and will result in vasodilation and hypotension. Benzodiazepines such as diazepam or midazolam are also effective sedatives in ruminant and camelid species. Opioids such as morphine or butorphanol will also provide sedation and analgesia. Of all the livestock species, sedation of swine is probably the most unpredictable and may require high doses to obtain the desired effect. Combinations of sedatives are often used to provide more consistent and effective sedation and analgesia, particularly in pigs. Anesthetic drugs vary in cost and are harder to obtain because many will be controlled substances. Pre‐euthanasia anesthesia (unconsciousness) is necessary before performing certain euthanasia procedures like the IV administration of KCl or ITh lidocaine. Ketamine is the most common anesthetic used in livestock. The cardiovascular and respiratory depressant effects of sedatives or general anesthesia have the potential to prolong the duration of subsequent euthanasia when using the injection of pentobarbital‐containing solutions. Clients who may be observing the procedure should be forewarned of this effect when using these drugs. In many instances, it may not be possible to safely restrain the animal simply with a halter or lariat or when an appropriate chute with a side opening is not available. In these cases, immobilization with sedation is required. A variety of drugs and drug combinations can be used for this, some of which are listed in Table 5.2. Xylazine is a common inexpensive sedative analgesic used in livestock. It provides reliable sedation and recumbency with relatively low doses and low cost. Detomidine is another α2‐adrenergic agonist sedative that could be used for sedation and restraint. However, Detomidine is more expensive and results in greater cardiorespiratory depression that may result in a prolonged response to lethal injection with barbiturates. Acepromazine is also an effective sedative in cattle but does not provide any significant analgesia. In addition, the hypotension resulting from inhibition of catecholamine‐mediated vascular tone may result in prolonged response to lethal injection. Combinations of xylazine, butorphanol, and ketamine (Ket–Stun protocols) and tiletamine/zolazepam, ketamine, and xylazine (TKX protocols) can be used for reliable standing and recumbent restraint in cattle. Such combinations can provide profound anesthesia and analgesia. In some instances, the animal may not be safely approached in order to administer sedation directly. Examples would be some range beef cattle, rodeo cattle, and bison. In these situations, the animals are generally excitable and may override simple sedation with just xylazine. A pole syringe or dart gun may be necessary for safe sedation in these animals. The drug chambers for pole syringes and dart guns are often limited in the volume that can be administered. The low volume required for TKX protocols is advantageous for anesthesia and recumbency in these excitable and potentially dangerous animals. Otherwise, euthanasia by gunshot at a longer range with a hunting rifle is appropriate. When using anesthesia drug combinations for the purpose of inducing unconsciousness, ensure the patient is ready before proceeding with a potentially painful euthanasia procedure. Table 5.2 Some suggested drugs and drug combinations for providing standing or recumbent sedation prior to euthanasia in cattle. Sedation is rarely necessary for the euthanasia of sheep and goats that are comfortable with human contact and handling. They are accustomed to manual restraint or with the use of a nylon, leather, or rope halter. For excitable animals, such as wild sheep and goats, sedatives, as used in cattle, can be used in sheep and goats with similar considerations. A list of several options for small ruminants is listed in Table 5.3. Table 5.3 Some suggested drugs and drug combinations for providing standing or recumbent sedation prior to euthanasia in small ruminants.
5
Livestock Euthanasia
5.1 Species‐Specific Considerations
5.1.1 Methods of Euthanasia
5.1.1.1 Euthanasia by Lethal Injection
5.1.1.2 Physical Methods of Euthanasia
5.2 Equipment and Handling
5.2.1 Supply Needs for Lethal Injection
5.2.2 Firearm Selection
Firearm
Animal type
Caliber
Bullet
Handgun or rifle
Calves, sheep, goats
.22
Soft or hollow point
5.5 mm
Pigs <100 kg
.22
Round nose
5.5 mm
Adult cattle (>24 months)
.22 magnum
Round nose
.357
Soft or hollow point
9 mm
Soft or hollow point
Horses
.357
Soft or hollow point
9 mm
Soft or hollow point
Adult bulls, pigs > 100 kg
.357
Round nose
9 mm
Round nose
Shotgun
Calves, sheep, goats
20, 16, or 12 gauge
#4, 5, or 6 birdshot
Pigs <100 kg
20, 16, or 12 gauge
#4, 5, or 6 birdshot
Adult cattle
16 or 12 gauge
Slug or #4 or 5 birdshot
Horses
16 or 12 gauge
Slug or #4 or 5 birdshot
Adult bulls, pigs > 100 kg
12 gauge
Slug or #4 or 5 birdshot
5.2.3 Captive Bolt
5.3 Pre‐Euthanasia Sedation and Anesthesia
5.3.1 Cattle
Drug or drug combination
Dose and route
Comments
Xylazine
0.01–0.05 mg/kg, IV
0.01–0.1 mg/kg, IM
Standing sedation. Dose‐dependent on the excitability of the animal.
0.1–0.2 mg/kg, IV or IM
Results in recumbency in most cattle. Higher doses can be used in anxious animals to assure recumbency prior to euthanasia.
Detomidine
0.002–0.02 mg/kg, IV or IM
Standing sedation. Dose‐dependent on the excitability of the animal.
Acepromazine
0.05–0.1 mg/kg, IV or IM
Will provide sedation but no analgesia. Marked hypotension may prolong euthanasia by injectable barbiturates.
Standing
Ket–Stun
Butorphanol
Xylazine
Ketamine
Combine for SC or IM injection (1:2:4 ratio)
0.01–0.025 mg/kg
0.02–0.05 mg/kg
0.04–0.10 mg/kg
Provides effective standing sedation and chemical restraint for most cattle. Anxious cattle may need a higher dose. Increasing xylazine increases the likelihood of recumbency.
Recumbent Ket–Stun
Butorphanol
Xylazine
Ketamine
Combine for SC or IM injection.
0.025 mg/kg
0.05 mg/kg
0.1 mg/kg
Routinely provides sedation and recumbency in most cattle. Onset in 3–10 min.
Xylazine–ketamine combination
Xylazine 0.05 mg/kg, IVKetamine 4.5 mg/kg, IV
Produces rapid anesthesia that will last for 15–20 min.
TKX–ruminant
Tiletamine
Ketamine
Xylazine
Reconstitute a 500 mg vial of tiletamine with 250 mg (2.5 mL) ketamine and 100 mg (1 mL) xylazine.
Administer at 1–1.5 mL/110 kg, IM. This equates to a dose of tiletamine 0.53 mg/kg, ketamine 0.53 mg/kg, and xylazine 0.04 mg/kg.
Recumbency in 5–10 min. The low volume makes this combination ideal for immobilizing intractable ruminants. It may be administered by syringe, with a pole syringe, or dart gun if needed. Relatively expensive.
5.3.2 Sheep and Goats
Drug or drug combination
Dose and route
Comments
Xylazine
0.01–0.05 mg/kg, IV
0.01–0.1 mg/kg, IM
Standing sedation. Dose‐dependent on the excitability of the animal.
0.1–0.2 mg/kg, IV or IM
Results in recumbency in most cattle. Higher doses can be used in anxious animals to assure recumbency prior to euthanasia.
Detomidine
0.002–0.02 mg/kg, IV or IM
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