Fig. 1.
Photographs showing MPMS100A-1 Samba201control unit (a), TSD175 ultraminiature optical pressure transducer (b), and the optic fiber probe (c).
4.
Sterile surgical drapes, gown, and gloves
5.
Hair Clipper
6.
Ophthalmic ointment
7.
Temperature-controlled heating pad
8.
Betadine
9.
Cotton-tipped applicators
10.
Scalpel handle with scalpel blades
11.
Retractors
12.
Dremel drill with 1-mm drill bit
13.
3-0 Dermalon suture
14.
Needle holder
15.
23-gauge needle tip
16.
Histoacryl
17.
Dental cement
18.
PTFE tube
3 Procedures
3.1 Preparation of Samba ICP Transducer
1.
Remove the cover of the optic fiber probe (diameter 0.42 mm, Samba Sensors, Biopac Systems, Inc, Fig. 49.1c).
2.
To protect the optical probe from direct mechanical influence by the enclosing brain tissue, the optic fiber probe is inserted into a 0.9-mm PTFE tube.
3.2 Preparation of Samba ICP Monitor System
1.
Connect the Samba transducer to the control unit. The transducer is factory calibrated. No further calibration is needed.
2.
Turn on the control unit. Need 1–2 min to stabilize the internal circuitry temperature.
3.
Set the “zero baseline” against ambient pressure.
4.
Connect the control unit to computer or data collection system.
3.3 Step-By-Step Procedures of ICP Monitoring
1.
Inject sodium pentobarbital (50 mg/kg, i.p.) to anesthetize the Sprague–Dawley (SD) rats (250–300 g).
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