Fig. 1.
(a) Findings in transmission electron microscopy of canine basilar artery. (b) Findings in scanning electron microscopy of canine basilar artery.
After perfusion fixation, the brain with arteries is taken (see Chap. 46). The artery to be evaluated is taken apart from the brain under an operative microscope or a substance microscope. For example, when SAH is produced by blood injection into the cisterna magna, the basilar artery that is subject to be examined is carefully taken from the pons. The artery taken apart is cut on length of 1–2 mm. And the rest of the artery can be used for light microscopy and scanning electron microscopy if necessary.
The specimen is fixed in the same solution as the perfusion fixation, and then post-fixed with 1% osmium tetroxide in buffer solution for 2 h, and dehydrated in a graded series of ethanol solutions. The specimen is bathed with propylene oxide and embedded in epoxy-resin. Ultra-thin section is obtained using an ultra microtome, and this section is stained with uranyl acetate and lead citrate.
2.
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Scanning electron microscopy (Fig. 1b):
The artery taken as described before is incised longitudinally under a surgical microscope when the luminal surface is observed (see Note 2).
The specimen is post-fixed with 1% osmium tetroxide in buffer (pH 7.4) for 90 min at 4°C, dehydrated in a graded ethanol series, dried at the critical point, and sputter-coated with gold.
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