Diagnostic Sampling

Diagnostic Sampling


• Hemolymph collection

image To assess physiologic parameters, hemolymph samples may be obtained.

image The small sample amount, not to exceed 10% of patient body weight, can make sampling difficult. In mollusks, hemolymph may be obtained from the heart or the cephalopod sinus by means of a capillary tube or a 25-gauge needle and syringe.

image To access the heart, a hole may be made in the shell using a dental drill.

image Alternatively, hemolymph can be sampled from the cephalopedal sinus in anesthetized patients.

image In bivalve mollusks, once the adductor muscle is transected and the left valve is removed, the heart can be found by its beating.

image Alternatively, one may file a notch in each valve to create an opening and then insert the needle into the adductor sinus through the adductor muscle.

image Another method for bleeding snails may be done without shell perforation. Locate the pneumostome (respiratory opening) as a landmark by watching for the appearance of bubbles.

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Jul 28, 2016 | Posted by in EXOTIC, WILD, ZOO | Comments Off on Diagnostic Sampling

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