Coombs’ Test Basic Information Definition Detection of erythrocyte associated immunoglobulins (IgG and IgM) and complement Synonym(s) May also be referred to as the direct antiglobulin test (DAT). Typical Normal Range (US units; SI units) Results are most commonly reported as being either positive or negative. Some laboratories may report dilutions at which the test was positive/negative. Dilutions of the test are performed to optimize the concentration of reagent in the presence of the patient’s erythrocytes. The dilutions at which a sample is positive do not correlate with the severity of disease. Physiology In the Coombs’ test, the patient’s washed erythrocytes are incubated with serial dilutions of a polyvalent Coombs’ reagent (contains a mixture of anti-IgG, IgM, and C3 complement protein antibodies). If immunoglobulins or complement proteins are present on erythrocytes, then cross linking and microscopically visible agglutination should occur. Causes of Abnormally High Levels Immune mediated hemolytic anemia (IMHA) results in a positive test. IMHA may be a primary autoimmune disease, secondary to drugs, infection, or neoplastic processes, or may be associated with the presence of alloantibodies in neonatal isoerythrolysis and blood transfusions.< div class='tao-gold-member'> Only gold members can continue reading. Log In or Register to continue Share this:Click to share on Twitter (Opens in new window)Click to share on Facebook (Opens in new window) Related Related posts: Aggressive Stallion Behavior Aflatoxin Toxicosis Biliary Atresia Black Widow Spider Toxicosis Stay updated, free articles. Join our Telegram channel Join Tags: Clinical Veterinary Advisor The Horse Jul 24, 2016 | Posted by admin in SMALL ANIMAL | Comments Off on Coombs’ Test Full access? Get Clinical Tree
Coombs’ Test Basic Information Definition Detection of erythrocyte associated immunoglobulins (IgG and IgM) and complement Synonym(s) May also be referred to as the direct antiglobulin test (DAT). Typical Normal Range (US units; SI units) Results are most commonly reported as being either positive or negative. Some laboratories may report dilutions at which the test was positive/negative. Dilutions of the test are performed to optimize the concentration of reagent in the presence of the patient’s erythrocytes. The dilutions at which a sample is positive do not correlate with the severity of disease. Physiology In the Coombs’ test, the patient’s washed erythrocytes are incubated with serial dilutions of a polyvalent Coombs’ reagent (contains a mixture of anti-IgG, IgM, and C3 complement protein antibodies). If immunoglobulins or complement proteins are present on erythrocytes, then cross linking and microscopically visible agglutination should occur. Causes of Abnormally High Levels Immune mediated hemolytic anemia (IMHA) results in a positive test. IMHA may be a primary autoimmune disease, secondary to drugs, infection, or neoplastic processes, or may be associated with the presence of alloantibodies in neonatal isoerythrolysis and blood transfusions.< div class='tao-gold-member'> Only gold members can continue reading. Log In or Register to continue Share this:Click to share on Twitter (Opens in new window)Click to share on Facebook (Opens in new window) Related Related posts: Aggressive Stallion Behavior Aflatoxin Toxicosis Biliary Atresia Black Widow Spider Toxicosis Stay updated, free articles. Join our Telegram channel Join Tags: Clinical Veterinary Advisor The Horse Jul 24, 2016 | Posted by admin in SMALL ANIMAL | Comments Off on Coombs’ Test Full access? Get Clinical Tree
